不同铁利用效率大豆品种在石灰性土壤中抗氧化酶活性的差异

Antioxidant enzyme activeties of soybean varieties with different iron efficiency growing in calcareous soil

关键词:  铁效率  大豆  抗氧化酶  酶活性  石灰性土壤

Key words:  iron efficiency  soybean  antioxidant enzymes  enzyme activity  calcareous soil

摘要:

为探明不同铁利用效率大豆品种抗氧化酶活性的差异，以铁效率差异显著的铁高效品种(‘吉育99’、‘吉育75’、‘长农15’、‘长农20’)和铁低效品种(‘吉农27’、‘吉育87’、‘吉育92’、‘吉育93’)大豆为材料，采用盆栽方式，种植在石灰性土壤中，测定不同铁利用效率的大豆农艺性状、籽粒含铁量、不同发育时期的叶绿素含量(SPAD)和抗氧化酶活性等指标。结果表明，铁高效与铁低效大豆品种株高、单株荚重、单株粒重、单株生物量和籽粒含铁量均存在显著差异；在石灰性土壤中，在三叶期和四叶期时铁高效品种大豆叶片的SPAD和SOD活性显著高于铁低效品种；在一叶期、二叶期，铁高效大豆品种叶片中的CAT活性显著高于铁低效品种；而POD活性各时期(V₁~R₂)无显著差异。铁高效大豆品种(‘吉育99’、‘吉育75’、‘长农15’、‘长农20’)在缺铁条件下通过维持较高的抗氧化酶活性来避免膜质过氧化导致的光合能力下降。

Abstract:

To illustrate the differences of different iron efficiency soybean varieties in antioxidant enzyme activity, iron efficient soybean varieties(‘jiyu 99', ‘jiyu 75', ‘changnong 15', ‘changnong 20')and iron inefficient(‘jinong 27', ‘jiyu 87', ‘jiyu 92', ‘jiyu 93')were taken as study material plants were grown in pots containing calcareous soil. The soybean agronomic traits and grain iron content and different development periods of chlorophyll content(SPAD)and antioxidant enzymes and other indicators were determined. The results showed that: There were significant differences in plant height, pod weight, grain weight, biomass and iron content in seeds between high and low efficiency soybean varieties. In calcareous soil, the SPAD and SOD activities in the leaves of soybean with high iron efficiency were significantly higher than those of soybean varieties with low iron efficiency the three-leaf stage and four-leaf stage. The CAT activity in the leaves of iron efficient soybean varieties was significantly higher than that of iron inefficient soybean varieties at the one-leaf and two-leaf stages. There was no significant difference in the POD activity at different stages(V₁-R₂). In conclusion, the iron-efficient soybean varieties(‘jiyu 99', ‘jiyu 75', ‘changnong15' and ‘Changnong 20')can avoid the decrease of photosynthetic capacity caused by membrane peroxidation by maintaining high antioxidant enzyme activity under the condition of iron deficiency.