外源NO缓解花生盐胁迫的效果及机理
投稿时间:2020-10-20    点此下载全文
引用本文:朱营营,张倩,董元杰.外源NO缓解花生盐胁迫的效果及机理[J].中国农业大学学报,2020,25(12):30-39
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朱营营 山东农业大学 资源与环境学院/土肥资源高效利用国家工程实验室, 山东 泰安 271018 1365867372@qq.com 
张倩 山东农业大学 资源与环境学院/土肥资源高效利用国家工程实验室, 山东 泰安 271018  
董元杰 山东农业大学 资源与环境学院/土肥资源高效利用国家工程实验室, 山东 泰安 271018 yuanjiedong@163.com 
基金项目:本研究由山东省农业重大应用技术创新项目(SD2019ZZ021);山东省自然科学基金(ZR2017MD010);泰山学者种业创新团队项目(2014)
中文摘要:为研究外源一氧化氮(NO)对盐胁迫下花生幼苗生长的影响,以‘山花11号’为供试材料,采用液体培养试验,测定外源NO供体硝普钠(SNP)250 μmol/L对正常生长(0 mmol/L NaCl,CK)和盐胁迫(100 mmol/L NaCl,T1和150 mmol/L NaCl,T2)处理的花生幼苗生长及生理指标。结果表明,正常生长条件下添加SNP,花生内源NO浓度升高对幼苗生长产生轻微毒害作用;盐胁迫下添加SNP能有效增强花生幼苗耐盐性,且外源NO对花生幼苗盐胁迫的缓解作用受外界盐浓度影响,花生遭受较高盐浓度胁迫时NO缓解膜脂过氧化损伤的效率更高,耐盐性更强;在本试验的浓度范围内,外源NO对150 mmol/L NaCl胁迫下的花生幼苗缓解效应更优。与T2处理相比,T5处理下的花生,叶片和根系的内源NO含量分别增加41.83%和35.45%。NO能显著提高花生体内抗氧化酶活性和渗透调节物质含量,叶片的SOD活性、POD活性、CAT活性和APX活性分别提升9.84%、64.82%、91.84%和26.09%,游离脯氨酸含量提升60.73%;降低体内丙二醛(MDA)和活性氧(ROS)的含量,使叶片电解质外渗率降低17.61%,有效减轻膜脂过氧化损伤;增加花生幼苗株高、鲜重、干重及叶绿素总含量,提高根系活力,有效缓解盐胁迫对花生幼苗生长的抑制作用。因此,正常条件下添加SNP不利于花生生长;但盐胁迫下添加SNP能显著提高花生苗期耐盐性,且外源NO缓解较高浓度盐胁迫对花生生长影响的效果更优。
中文关键词:花生  一氧化氮  盐胁迫  生理特性  机理
 
Effects of exogenous nitric oxide on the physiological characteristics of peanut seedlings under salt stress
Abstract:In order to study the effect of exogenous nitric oxide(NO)on the growth of peanut seedlings under salt stress, ‘Shanhua 11' was taken as the test material. A liquid culture test was used to determine the exogenous NO donor sodium nitroprusside(SNP)250 μmol/L on the growth and physiological indexes of peanut seedlings treated with normal growth(0 mmol/L NaCl, CK)and salt stress(100 mmol/L NaCl, T1 and 150 mmol/L NaCl, T2). The results showed that: Under normal growth conditions, the increase of endogenous NO concentration in peanuts displayed a slight toxic effect on seedling growth; The addition of SNPs under salt stress could effectively improve the salt tolerance of peanut seedlings, and the alleviating effect of exogenous NO on peanut salt stress is affected by the external salt concentration. When peanut was subjected to higher salt concentration stress, the NO relieved membrane lipid peroxidation more quickly and had better salt tolerance. Within the concentration range of this experiment, the exogenous NO had better mitigating effect on peanut seedlings under 150 mmol/L NaCl stress. Compared with T2 treatment, the content of endogenous NO in leaves and roots of peanut treated with T5 increased by 41. 83% and 35. 45%, respectively. NO significantly increased the antioxidant enzyme activity and osmotic substance content in peanuts. The SOD, POD, CAT and APX activities of leaves were increased by 9. 84%, 64. 82%, 91. 84% and 26. 09%, respectively. Proline content was increased by 60. 73%. The accumulations of malondialdehyde(MDA)and reactive oxygen species(ROS)in the body were reduced, the electrolyte extravasation rate of leaves was decreased by 17. 61%, and the membrane lipid peroxidation damage was effectively reduced; the peanut seedling plant height, fresh and dry weight and total chlorophyll content were increased; The root vigor of peanut was increased. It could effectively relieve the inhibitory effect of salt stress on the growth of peanut seedlings. In conclusion, the addition of SNP under normal conditions was not conducive to peanut growth. However, the addition of SNP under salt stress significantly improved the salt tolerance of peanut seedlings, and the effect of exogenous NO on alleviating the effects of higher concentrations of salt stress on peanut growth was better.
keywords:peanut  nitric oxide  salt stress  physiological characteristics  mechanism
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