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| 褪黑素对内蒙古绒山羊皮肤上皮细胞增殖及wnt10b和β-catenin基因表达的影响 |
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吴子贤1,2,吴静3,宫文典1,卢泽宇1,赵飞飞1,刘俊阳1,杨德智1,张燕军1,苏蕊1,赵艳红1*,刘佳森2*
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| (1.内蒙古农业大学 动物科学学院, 呼和浩特 010018;2.内蒙古自治区农牧业科学院 畜牧研究所, 呼和浩特 010031;3.内蒙古乌兰察布市商都县职业技术学校, 内蒙古 乌兰察布 013450) |
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| 摘要: |
| 为研究褪黑素对内蒙古绒山羊皮肤上皮细胞生长的作用模式，将褪黑素浓度设置为0、100、300、600和900 pg/mL 5个试验组，利用CCK-8法检测各组皮肤上皮细胞增殖情况，实时荧光定量PCR检测Wnt10b和β-catenin基因的表达，ELISA检测Wnt10b及β-catenin蛋白的表达。结果表明:300 pg/mL褪黑素对皮肤上皮细胞增殖效果最佳(P<0.05)，Wnt10b和β-catenin的mRNA和蛋白质表达量极显著高于其他组(P<0.01)。综上，300 pg/mL的褪黑素可显著促进皮肤上皮细胞增殖，且显著提高Wnt10b和β-catenin的表达。 |
| 关键词: 内蒙古绒山羊 褪黑素 皮肤上皮细胞 细胞增殖 Wnt10b β-catenin |
| DOI：10.11841/j.issn.1007-4333.2021.01.07 |
| 投稿时间：2019-11-26 |
| 基金项目:国家自然科学基金项目(31860628、31460588、31560624)；内蒙古自然基金项目(2020LH03020、2015MS0381、2017MS0304)；内蒙古农牧业科技创新基金项目(2017CXJJM01-3) |
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| Effects of melatonin on the proliferation of skin epithelial cells and expressions of Wnt10b and β-catenin genes in Inner Mongolian cashmere goats |
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WU Zixian1,WU Jing1,GONG Wendian1,LU Zeyu1,ZHAO Feifei1,LIU Junyang1,YANG Dezhi1,ZHANG Yanjun1,SU Rui1,ZHAO Yanhong1*,LIU Jiasen2*
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| (1.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;2.Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;3.Inner Mongolia Wulanchabu City Shangdu County Vocational and Technical School, Ulanqab 013450, China) |
| Abstract: |
| In order to study the effect of melatonin on the growth of skin epithelial cells of Inner Mongolia cashmere goats, five test groups were set at 0, 100, 300, 600 and 900 pg/mL of melatonin. CCK-8 method was used to detect the proliferation of skin epithelial cells. Quantitative PCR was to detect the expression of Wnt10b and β-catenin gene, and ELISA was used to detect the expression of Wnt10b and β-catenin protein. The results showed that 300 pg/mL melatonin had the best effect on the proliferation of skin epithelial cells(P<0. 05), and the mRNA and protein expressions of Wnt10b and β-catenin were significantly higher than those in other groups(P<0. 01). In summary, 300 pg/mL melatonin can significantly promote the proliferation of skin epithelial cells and increase the expressions of Wnt10b and β-catenin. |
| Key words: Inner Mongolian cashmere goats melatonin skin epithelial cells cell proliferation Wnt10b β-catenin |
