| 摘要: |
| 为稳定并提高萨福克羔羊卵泡发育效率,通过肌肉注射FSH和PMSG的方法,对可能影响萨福克羔羊激素诱导效果如羔羊日龄、体重、健康状况和季节等因素进行研究。结果显示:1)5~6周龄萨福克羔羊经激素处理后的平均卵泡数(114.18 vs 22.17)、回收的卵母细胞数(82.54 vs 12.83)以及可用卵母细胞数(72.79 vs 10.83)极显著高于8~9周龄组(P<0.01);2)体重在8~18 kg之间的5~6周龄羔羊,不影响处理效果(P>0.05);3)有疾病史的羔羊处理效果显著低于健康羔羊组(P<0.01);4)春、秋季节对羔羊卵泡发育及回收的卵母细胞数没有影响(P>0.05),但春季羔羊卵母细胞体外受精卵裂率(54.31% vs 73.93%)和囊胚率(21.34% vs 35.73%)显著低于秋季(P<0.05);5)秋季羔羊胚胎移植受胎率(51.33% vs 26.07%)显著高于春季(P<0.05);6)移植早期胚胎或囊胚期胚胎对受胎率没有影响(P>0.05)。选择5~6周龄、体重在8~18 kg之间、无疾病史的萨福克羔羊,能显著提高卵泡发育效率;秋季羔羊卵母细胞发育能力显著高于春季;相同季节内,移植早期胚胎或囊胚期胚胎受胎率间没有差异。 |
| 关键词: 外源激素 萨福克羔羊 卵母细胞 体外受精 胚胎移植 |
| DOI：10.11841/j.issn.1007-4333.2015.04.19 |
| 投稿时间：2014-11-03 |
| 基金项目:国家自然基金资助项目(U1203381);新疆自治区科技计划项目(201111113);新疆自治区科技支疆项目(201291147);国家转基因重大专项(2014ZX08008-003);山西省生态站项目 |
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| Study on the induced development effect of Suffolk lamb follicular |
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WANG Li-qin1, HE Zong-lin2, LIN Jia-peng1, WU Yang-sheng1, HUANG Jun-cheng1
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| (1.Key Laboratory of Grass Livestock Reproduction&Breed Biotechnology Ministry of Agriculture, Animal Science Academy of Xinjiang, Urumqi 830000, China;2.Akesu Animal Disease Control Diagnosis Center, Akesu 843000, China) |
| Abstract: |
| In order to stabilize and improve the lamb oocytes in vitro production efficiency, the influence of lamb age, weight, health status, season and other factors on Suffolk lamb hormone was studied.The results showed that:1) The average number of follicles of the 5-6 weeks aged Suffolk lambs was significantly higher than those of 8-9 weeks aged group (P<0.01) after treated by hormone(114.18 vs 22.17)、oocytes recovered(82.54 vs 12.83)and usable oocytes(72.79 vs 10.83);2) There is no significant effect for those lambs weighed between 8-18 kg(P > 0.05);3) The effects were significantly lower in those lambs with a history of disease than those healthy lambs (P<0.01);4) There is no significant season effect (Spring vs.autumn) on lamb follicular development and oocyte recovery (P>0.05).However, the cleavage rate(54.31% vs 73.93%)and blastocyst rate(21.34% vs 35.73%) in the spring was significantly lower than in autumn(P<0.05);5)Autumn lamb embryo transfer pregnancy rate was significantly higher than that in spring (51.33% vs 26.07%, P<0.05).6)The transplantation of 2-8 cells or blastocyst had no effect on pregnancy rate(P>0.05).Conclusion:The Suffolk lambs aged 5-6 weeks, weighed 8-18 kg, with no history disease, can significantly improve the efficiency of follicular development.Autumn lamb developmental capacity of oocytes was significantly higher than that in spring.In the same season, there was no difference between the transplanted embryonic or blastocyst stage embryo conception rate. |
| Key words: exogenous hormones Suffolk lamb oocytes in vitro fertilization embryo transfer |
