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| 复烤烟叶异地醇化过程中生物活性的变化 |
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汪长国1,2, 李宁1,2, 寇明钰1,2, 冯广林1,2, 杨文敏1,2, 吴艳1,2, 刘林1,2, 戴亚1,2, 张燕3
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| (1.减害降焦四川省重点实验室,成都 610066;2.川渝中烟工业有限责任公司,成都 610066;3.云南省烟草质量监督检测站,昆明 650106) |
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| 摘要: |
| 以复烤烟叶为试验材料,研究了异地醇化过程中生物活性的变化。结果表明:复烤烟叶异地醇化过程中,醇化初期叶面微生物数量和生物酶活性迅速上升,之后随着醇化的进行而逐渐下降;中部烟叶叶面微生物数量总体大于上部和下部烟叶,并可检出蛋白酶活性,而上部和下部烟叶均未能检出其活性,纤维素酶和淀粉酶活性均为中部>上部>下部烟叶,而蔗糖酶活性为下部>中部>上部烟叶;同等级复烤烟叶存放于西昌烟叶仓库的生物活性水平高于成都烟叶仓库。 |
| 关键词: 复烤烟叶 醇化 微生物 酶 生物活性 |
| DOI：10.11841/j.issn.1007-4333.2013.02.016 |
| 投稿时间：2012-09-07 |
| 基金项目:四川省立项项目(2009JY0153) |
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| Changes of biological activity of flue-cured tobacco leaves from different places aging |
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WANG Chang-guo1,2, LI Ning1,2, KOU Ming-yu1,2, FENG Guang-lin1,2, YANG Wen-min1,2, WU Yan1,2, LIU Lin1,2, DAI Ya1,2, ZHANG Yan3
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| (1.Sichuan Key Laboratory for Harmful Component and Tar Reduction in Cigarette,Chengdu 610066,China;2.China Tobacco Chuanyu Industrial Co.,Ltd,Chengdu 610066,China;3.Yunnan Provincial Tobacco Quality Supervision and Inspection Station,Kunming 650106,China) |
| Abstract: |
| The changes of biological activity during different place aging were studied using flue-cured tobacco leaves as the experimental materials.The results showed that during different place aging the amount of microorganisms and enzyme activity of flue-cured tobacco leaves grew rapidly during the early period of aging,but gradually reduced along with the aging.The amount of microorganisms on central flue-cured tobacco leaves was generally higher than those from the upper and bottom flue-cured tobacco leaves.The activity of protease in central flue-cured tobacco leaves was also detected, but no activity was identified from the upper and bottom flue-cured tobacco leaves.The order for the activity of cellulase and amylase was central>upper>bottom flue-cured tobacco leaves,while the order for the activity of sucrase was bottom>central>upper flue-cured tobacco leaves.It was found that the biological activity of the same degree flue-cured tobacco leaves in the Xichang tobacco leaves warehouse was higher than that of the Chengdu tobacco leaves warehouse. |
| Key words: flue-cured tobacco leaves aging microorganism enzyme biological activity |
