| 摘要: |
| 探讨不同光照强度条件下忽地笑鳞茎中石蒜碱和加兰他敏含量变化,为忽地笑规范化栽培提供科学依据。采用HPLC方法测定了100%自然光、50%遮荫和85%遮荫条件下忽地笑鳞茎中石蒜碱和加兰他敏含量。3种不同光强下忽地笑鳞茎中石蒜碱和加兰他敏含量均表现为:50%遮荫>85%遮荫>100%自然光,其中在50%遮荫条件下其含量均最高,与100%自然光的相比,分别提高了35.88%和20.56%,差异均达到极显著水平(P<0.01)。稳定性及回收率实验结果表明:HPLC法测定忽地笑鳞茎中石蒜碱和加兰他敏含量效果好,稳定性强,方法可靠;适度遮荫(如50%遮荫)可以提高忽地笑鳞茎中石蒜碱和加兰他敏含量。忽地笑适宜在一定遮荫环境下栽培。 |
| 关键词: 忽地笑 遮荫 HPLC 石蒜碱 加兰他敏 |
| DOI：10.11841/j.issn.1007-4333.2012.04.010 |
| 投稿时间：2012-03-02 |
| 基金项目:湖南省高校创新平台开放基金项目(11K051);湖南省科技计划重点项目(2009FJ2008)第一作者:全妙华,副教授,主要从事植物生理生化等研究,E-mail:hhqmh100@163.com |
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| Effect of shading on contents of lycorine and galantamine in the bulb of Lycoris aurea |
|
QUAN Miao-hua, SHE Chao-wen, OU Li-jun, CHEN Dong-ming
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| (Department of Life Sciences/Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources/Key Laboratory of Hunan Higher Education for Hunan-Western Medicinal Plant and Ethnobotany, Huaihua University, Huihua 418008, China) |
| Abstract: |
| Changing of lycorine and galantamine contents in the bulb of Lycoris aurea under different light intensity conditions were analyzed in order to provide scientific information for cultivation of Lycoris aurea.Contents of lycorine and galantamine in the bulbs under 100% natural light,50% shading and 85% shading were determined by HPLC.The contents of lycorine and galantamine in the bulbs under the three different conditions were as following:50% shading >85% shading >100% natural light.The contents of lycorine and galantamine under 50% shading were both the highest,and increased 35.88% and 20.56% compared with those under 100% natural light,respectively with high significant level (P<0.01).The results determined by HPLC were highly repeatable,indicating that the method used in this study was reliable.Proper shading,such as 50% shading,increased contents of lycorine and galantamine in the bulb of Lycoris aurea.Thus Lycoris aurea was suitable for cultivation under moderate shading. |
| Key words: Lycoris aurea shading HPLC lycorine galantamine |
