| 摘要: |
| 本研究以小麦品种聊大1号为试验材料,研究UV-B辐射对聊大1号苗期叶片蛋白质组的影响。双向电泳分析发现,UV-B辐射处理和未处理的聊大1号有15个蛋白差异点,经质谱分析成功鉴定出6个蛋白差异点:其中1个为Rubisco大亚基(碳水化合物代谢);2个为参与翻译类的蛋白;2个为具有运输类功能的蛋白;1个为质子-ATP酶α亚基。这些结果可为小麦抗UV-B辐射机理研究和抗性育种提供依据。 |
| 关键词: 小麦 苗期 UV-B辐射 蛋白质组 双向电泳 |
| DOI：10.11841/j.issn.1007-4333.2012.02.006 |
| 投稿时间：2011-11-11 |
| 基金项目:国家自然科学基金资助项目(31071412); 国家"973"计划项目(2009CB118300) |
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| Analysis of wheat seeding leaf proteome after UV-B radiation |
|
HUANG Xiu-wen1, ZHANG Yan-ju1, FENG Qin1, CHEN Wei-xia1, ZHANG Wen-hui2, LI Bao-yun1
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| (1.College of Agronomy and Biotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture/Key Laboratory of Crop Heterosis & Utilization of Ministry of Education, China Agricultural University, Beijing 100193, China;2.College of Life Science, Liaocheng University, Liaocheng 252059, China) |
| Abstract: |
| In order to investigate the results of UV-B radiation on the accumulation of proteins in wheat seeding leaves,the cultivar LiaoDa 1 was subjected to UV-B radiation stress.Compared with control samples,the UV-B radiation stressed samples accumulated significantly higher amounts of protein.These proteins were separated by two-dimensional electrophoresis (2-DE),visualized by silver staining and analyzed by Image Master.It revealed 15 differentially expressed polypeptides,6 of which were successful identified by MALDI-TOF,including a Rubisco large subunit,an ATP synthase CF1 alpha chain,proteins involved in protein translation and transportation.These results might provide information for further UV-B radiation stress study in wheat. |
| Key words: wheat seeding leaf UV-B radiation proteome 2-DE |
