| 摘要: |
| 快速高效的DNA提取方法是玉米大规模分子育种的第一步,因此,需要发展一种快速提取玉米基因组DNA的方法,以满足我国中小型实验室的需要。本研究介绍了一种可用于玉米大规模基因组DNA提取的方法:先利用改良碱煮法提取玉米籽粒小块胚乳的DNA,进行基因型鉴定,对大群体进行筛选,再利用电钻磨样及简化的CTAB法快速提取玉米苗期叶片的DNA,进行后期的研究。这种方法使得玉米大群体的筛选和研究简单化、快速化、常规化,可广泛应用于玉米分子育种。 |
| 关键词: 玉米 大群体 胚乳 碱煮法 快速提取 |
| DOI：10.11841/j.issn.1007-4333.2011.06.005 |
| 投稿时间：2011-04-11 |
| 基金项目:国家"973"计划项目(2007CB109001) |
|
| A rapid DNA extraction method for large maize populations |
|
GAO Yu-feng, ZHANG Pan, HAO Xiao-min, YAN Jian-bing, LI Jian-sheng, YANG Xiao-hong
|
| (National Maize Improvement Center of China/Key Laboratory of Crop Genomics andGenetic Improvement, China Agricultural University, 100193 Beijing, China) |
| Abstract: |
| Maize is one of the most important crops in China.With the development of molecular biology,molecular breeding is becoming one of the hot spots in maize genetics and improvement.The rapid method of DNA extraction with high efficiency is the first step in large-scale molecular breeding.Therefore,developing a rapid DNA extraction method for large maize populations is necessary to meet the need of the small labs in China.In this study,a new method of extracting DNA for genotyping large populations was developed.Firstly,kernel endosperm tissue in maize was used to extract DNA by using the improved alkali-boiling method;secondly,tender leaf tissue in maize was used to extract DNA by utilizing electric drill milling and simplified CTAB method.These two methods were combined to prepare DNA for genotyping before and after planting.Compared with the conventional ways of genotyping in large populations,this new method with higher efficiency and lower costs could be widely used in maize molecular breeding. |
| Key words: maize large populations endosperm alkali-boiling method rapid extraction |
