| 摘要: |
| 为探讨丁香属植物种间、欧丁香品种间以及丁香属与女贞属之间的亲缘关系,本研究使用8对选择性扩增引物对丁香属14个种、欧丁香种的37个品种和女贞属3个种进行了AFLP分析。利用Ntsyspc软件对扩增结果进行分析,以遗传相似性系数为基础,计算Nei遗传距离并采用UPGMA法进行聚类。结果表明:AFLP扩增共产生447条带,其中多态性条带395条,多态率为88.4%,各样品间的遗传距离在0.01~1.44之间。聚类结果与传统的分类结果基本一致,不同之处在于:部分欧丁香品种与顶生花序系的种聚类为一个分支;短花冠管组的种聚类于长花冠管组的内部;长花冠管组的种内部巧玲花系与顶生花序系的种聚类有交叉现象;女贞属植物聚类于丁香属植物的内部。据此推断,欧丁香品种的育种过程中有顶生花序系的种的加入;丁香属植物的分类应该去除组的划分;巧玲花系与顶生花序系的亲缘关系比较近;丁香属植物不是一个自然的单系类群,女贞属与丁香属之间的起源与分类学地位有待于进一步的研究。 |
| 关键词: 丁香种 欧丁香品种 AFLP 多样性 |
| DOI：10.11841/j.issn.1007-4333.2011.05.009 |
| 投稿时间：2011-01-05 |
| 基金项目:北京市教委科技计划面上项目(KM200510020003) |
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| Analysis of the phylogenetic relationship of Syringaby AFLP technique |
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GAO Hong-xiao1, YANG Kai2, LIU Jian-bin1
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| (1.Landscape Design & Forestry College,Beijing University of Agriculture,Beijing 102206,China;2.Key Laboratory of Agricultural New Technology and Application in Beijing,Beijing University of Agriculture,Beijing 102206,China) |
| Abstract: |
| To explore the relationships among Syring vulgaris,species of Syringa,Syringa and Ligustrum,eight EcoRⅠ/MseⅠ primer combinations were used for AFLP molecular marker technique.The genetic diversity of 14 Syringa species,37 cultivars of S.vulgaris and 3 Ligustrum species were evaluated.NTSYS was used to compute the genetic distance and UPGMA for clustering analysis.The results showed that about 447 discernable bands were obtained,395 being polymorphic with the percentage of 88.4%.The genetic distance computed by NTSYS was from 0.01 to 1.44.There were some differences between the clustering analysis and traditional taxonomic method,in which some Syring vulgaris were clusted with Ser.villosae,sect Ligustrina with Ser.syringa,species in Ser.villosae and Ser.pubescente,the Ligustrum species of S.pinnatifolia and S.microphylla.It implied that Ser.villosae participated the breeding process of some Syringa vulgaris.The classification of Syringa should break into two sects divide.Ser.villosae had a close relationship with Ser.pubescente.Syringa had a complex phylogenetic relationship.The further study is required for the origin and classification relationship of Syringa and Ligustrum. |
| Key words: Syringa species cultivars of S.vulgaris AFLP diversity |
