| 摘要: |
| 本研究在苗期对小麦品种Holdfast进行抗病性鉴定和温敏微效基因检测,并构建Holdfast和铭贤169的杂交群体,对其成株抗条锈病基因进行遗传分析。结果表明:Holdfast苗期常温下对中国流行小种CYR29、CYR31、CYR32和CYR33高度感病,高温下可诱导温敏微效基因表达;成株期大田条件下Holdfast对CYR32免疫,由1对显性和2对隐性基因互补控制。综合已有研究结果确认,Holdfast至少含有2对显性主效全生育期抗病基因、1对显性成株抗病基因和2对隐性温敏微效抗病基因。建议将其作为抗源在育种中加以利用。 |
| 关键词: 小麦 条锈病 成株抗病基因 遗传分析 |
| DOI：10.11841/j.issn.1007-4333.2011.05.001 |
| 投稿时间：2011-01-23 |
| 基金项目:农业公益性行业科研专项经费项目(200903035); 中国博士后科学基金特别资助项目(200902156); 四川省财政育种工程青年基金项目(2008QNJJ-013) |
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| Genetic analysis of resistance to stripe rust inwheat cultivar Holdfast |
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ZHANG Zhen-yu1,2, FENG Jing1, BAI Yu-lu2, LIN Rui-ming1, PENG Yun-liang2, XU Shi-chang1
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| (1.State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;2.Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China) |
| Abstract: |
| The wheat cultivar Holdfast introduced form England,was reported to be durably resistant to stripe (yellow) rust (Puccinia striiformis).In this work,Holdfast was evaluated for seedlings in greenhouse and adult stage in field,and also tested temperature sensitive gene.The genetic analysis of the cross between Holdfast and the susceptible cultivar Ming Xian 169 was conducted to identify the resistance character. The results showed that Holdfast was susceptible to the Chinese popular race CYR29、CYR31、CYR32 and CYR33 of Puccinia striiformis in seedlings, and had sensitive resistance to the race CYR32 in high temperature. The genetic analysis showed that the resistance to the race CYR32 was controlled by one dominant gene and two recessive genes complementarily. Considering the previous studies, it was confirmed that Holdfast had two dominant major genes in all stages, one dominant gene in adult and two recessive temperature-sensitive genes at least. Our results showed that Holdfast could be utilized in wheat resistance breeding. |
| Key words: wheat stripe (yellow) rust adult resistance gene genetic analysis |
