| 摘要: |
| 应用双向凝胶电泳技术,结合含水率及膜脂过氧化物测定等方法,研究镉(Cd)和1,2,4-三氯苯(TCB)污染对扬麦13幼苗生长的影响。结果表明:Cd和TCB污染造成扬麦13幼苗含水率降低,导致膜脂过氧化。Cd和TCB污染对小麦叶片中的蛋白质组分也有较大影响:与对照相比,污染处理后的叶片蛋白组分中检测出12个TCB胁迫诱导蛋白和10个Cd胁迫诱导蛋白,其中有些蛋白是TCB特异性诱导蛋白,也有一些蛋白为Cd2+胁迫特异性诱导蛋白。这些诱导蛋白的产生可能是扬麦13对Cd或TCB污染的一种适应性响应。 |
| 关键词: 小麦 镉 1,2,4-三氯苯 蛋白质组 污染 |
| DOI：10.11841/j.issn.1007-4333.2011.03.008 |
| 投稿时间：2010-09-26 |
| 基金项目:蚌埠学院自然科学重点项目(2010ZR02zd); 江苏省作物栽培生理重点实验室开放课题(K09009) |
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| Response of Cd and 1,2,4-Trichlorobenzene pollutantson growth of wheat seedlings |
|
DING Yan1, GE Cai-lin2, WANG Ze-gang2, DU Qing-cai1
|
| (1.Department of Chemistry and Environmental Engineering,Bengbu College,Bengbu 233000,China;2.College of Biological Sciences and Biotechnology,Yangzhou University,Yangzhou 225009,China) |
| Abstract: |
| The effects of wheat seedlings to Cd and 1,2,4-trichlorobenzene (TCB) pollutants were investigated by methods of Two-dimensional Electrophoresis,combined with water content,and lipid peroxide determination.The results showed that Cd and TCB pollutants inhibited the growth of leaves and roots which ledding to water deficit and causing lipid peroxidation in wheat s seedlings.Moreover,Cd and TCB stress had significant effect on global proteome in wheat leaves.Comparing with the contrast,we found that there were 12 stress inducible proteins of TCB,and 10 stress inducible proteins of Cd.The appearence of these induced proteins may be the important mechanism for Yangmai No.13 adapting to Cd and TCB pollutants. |
| Key words: wheat Cd2+ TCB proteome pollutant |
