| 摘要: |
| 为了给更好地研究神经元病理变化提供物质材料,对新生大鼠大脑皮质神经元进行体外分离和培养。无菌操作取新生大鼠的大脑皮质,采用机械法和胰蛋白酶消化法相结合的方法分离细胞,制备单层细胞悬液接种培养板;5-氟脱氧尿嘧啶处理等方法纯化神经元,甲苯胺蓝染色法及抗NSE单克隆抗体对纯化后的神经元进行鉴定。结果表明,培养的神经元生长良好,形态正常,纯度高达90%以上。采用本方法培养的神经元可作为深入研究中枢系统疾病的细胞模型材料。 |
| 关键词: 大鼠 大脑皮质 神经元 培养 胰蛋白酶 |
| DOI：10.11841/j.issn.1007-4333.2007.03.047 |
| 修订日期：2006-09-12 |
| 基金项目:北京市科委科研项目;高等学校博士学科点专项科研项目 |
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| Separation and cultivation of cortical neuron cell from a new rat |
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| Abstract: |
| A primary cortical neuron was separated and cultured from a new rat to provide a basal material for detailed study of pathological changes in the central nervous system.We obtained the cerebral cortex of the new rat by aseptic operation.Trypsinization and 5-fluorodeoxyuridine was used for harvesting and processing of the neurons.Neurons were identified by Toluldine blue dyeing and symbolic enzyme NSE.The cultured nerve cell developed satisfactorily with a purity >90%,hence,it can be used for the next research as a model cell. |
| Key words: rat,cerebral cortex,neuron,culture,trypsin |
