| 摘要: |
| 为增强肉牛的繁殖力,应用本实验室构建的抑制素真核融合表达质粒pCISI,提纯后辅以不同的免疫佐剂注入96头母黄牛体内,采用AMI-900兽用B超对其中84头发情牛进行卵泡检测,并对其中78头妊娠牛进行早期胚胎数目检测。结果发现:抑制素质粒pCISI能促进双大卵泡发育(80.95%,68/84),诱发排双卵(60.71%,51/84),诱导黄牛孪生(41.03%,32/78);普鲁卡因佐剂(48.78%)比脂质体(32.43%)效果明显。研究表明,抑制素基因免疫能有效提高黄牛的繁殖率。 |
| 关键词: 抑制素pCISI,基因免疫,黄牛,排卵,双胎 |
| DOI：10.11841/j.issn.1007-4333.2006.03.059 |
| 投稿时间：2005-11-07 |
| 基金项目:国家自然科学基金资助项目(30270959)，南京农业大学校级青年创新基金资助(y200411) |
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| Influence of inhibin gene immunization on twinningin cattle |
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| Abstract: |
| To improve the fertility of cattle,the fusion expressing plasmid of inhibin pCISI,which was designed and extracted in our laboratory,was used to immune the 96 female cattle.Besides,the AMI-900 ultrascanner was adopted to examine the follicles of 84 ruttish cattles and the embryos of 78 pregnant cattles.The results showed that inhibin pCISI could cause two big follicles development (80.95%,68/84) and induce two mature follicles bringing out(60.71%,51/84).Meanwhile,inhibin pCISI could induce 41.03%(32/78)cattle twinning.The procaine had obviously a better effect than the liposome(48.78% vs.32.43%).The research proved that inhibin gene immunization could effectively improve the reproduction rate of cattles. |
| Key words: inhibin pCISI,gene immunity,cattle,ovulate,twinning |
