| 摘要: |
| 普通番茄和转反义LeEIL2基因番茄不同成熟度果实研究结果表明,转反义LeEIL2基因番茄有效抑制了LeEIL2基因的表达,其果实和叶片表现出与普通番茄不同的生理特性:采后果实的呼吸强度和乙烯释放量受到抑制,离体叶片的伤乙烯释放量明显降低;果实中ACC合成酶与ACC氧化酶活性以及ACC含量明显下降,从而抑制了乙烯的生物合成途径,延缓了果实的成熟衰老进程。 |
| 关键词: 转基因,番茄,乙烯,生理特性 |
| DOI：10.11841/j.issn.1007-4333.2006.01.013 |
| 投稿时间：2005-03-08 |
| 基金项目:国家自然科学基金资助项目(30270934,30371004,30430490) |
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| Some characters of post harvest physiology of antisense LeEIL2 transgenic tomato fruit |
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| Abstract: |
| Fruits and leaf of anti-sense LeEIL2 tomato((Lycopersicon esculentum Mill.cv.Zhong Shu 4th) shown differences in ethylene biosynthesis and fruit ripening.Compared with CK,respiration rate and ethylene production rate in anti-sense LeEIL2 tomato fruit were inhibited;leaf wound ethylene from anti-sense tomato decreased to 41%.Further research confirmed that ACS and ACO activities in anti-sense LeEIL2 tomato fruit decreased dramatically,which resulted in ACC content decrease,ethylene biosynthesis inhibition and eventual fruit ripening delay. |
| Key words: transgenic,tomato,ethylene,ACC |
