| 摘要: |
| 采用直接比色法测定板蓝根中靛蓝、靛玉红的质量分数。对靛蓝、靛玉红在240～700nm波段进行扫描，确定其最大吸收波长分别为601和531nm。分别通过单因素试验和正交试验，确定提取靛蓝、靛玉红的最佳工艺参数。结果表明，乙醇体积分数为75％时，靛蓝和靛玉红的提取物得率最高；提取时间为4h时，靛蓝和靛玉红的提取物得率最高。正交试验确定的最佳提取工艺参数为：药材粒度40目，提取时间4h，乙醇体积分数75％，此时，提取物得率达1．74%。 |
| 关键词: 板蓝根 靛蓝 靛玉红 提取技术 质量分数 测定技术 单因素试验 正交试验 |
| DOI：10.11841/j.issn.1007-4333.2003.06.144 |
| 修订日期：2003-06-20 |
| 基金项目:国家“九五”重点攻关专题 |
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| Extraction and determination of mass ratio of Indigo and Indirubin in Indigowoad root |
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| Abstract: |
| Extraction and determination of mass ratio of Indigo and Indirubin in Indigowoad root were studied. Indigo and Indirubin were scanned from 240 to 700*!nm. From the spectrogram we can see that the largest absorbing wavelength of Indigo and Indirubin was 601 and 531*!nm respectively. The best parameters of technology have been determined through single factor and orthogonal test on extracting indigo and Indirubin respectively, results showed that ethanol with a volume ratio of 75% was suitable solvent to extract Indigo and Indirubin and 4 hours was the proper time to extract Indigo and Indirubin. Through orthogonal test the best parameters of technology were obtained. The results are as the following: the herbal granularity is 40 eye, extraction time is 4*!h, ethanol with a volume ratio of 75%. On this condition, the extracting rate was up to 1.74%. |
| Key words: indigowoad root,indigo,indirubin,mass ratio,extraction,determine |
