| 摘要: |
| 用100条10碱基随机引物，以普通小麦中国春，中间偃麦草为材料进行RAPD分析，筛选到一个偃麦草染色体组特异引物OPF03，并从中间偃麦草中克隆了该引物的特异DNA片段OPF031291。将该片断与比萨偃麦草中的OPF031296（GenBank序号U43516）比较，同源性为88％。根据OPF031291的序列，设计了2对SCAR引物，利用OPF03和引物P3，P4对普通小麦，普通小麦－中间偃麦草的部分双二倍体，长穗偃麦草，中间偃麦草，小麦－二倍体长穗偃麦草代换系，附加系共6类材料进行了RAPD和SCAR分析，发现RAP标记OPR031291没有出现在含E^e染色体组的材料中，而标记SCAR982则出现于所有含E染色体组的材料中。说明，根据E^b染色体组特异RAPD标记转化的SCAR标记，可以同时检测小麦背景下的E^e染色体。 |
| 关键词: 偃麦草 E染色体组 特异RAPD SCAR标记 |
| DOI： |
| 修订日期：2002-02-25 |
| 基金项目:国家自然科学基金重点资助项目 (39930 110 )，北京市自然科学基金重大资助项目 (6 990 0 0 1) |
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| Establishment of E-Genome-Specific RAPD and SCAR Markers for Thinopyrum spp. |
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| Abstract: |
| A total of 100 decamer oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis on Triticum aestivum var. Chinese Spring and Thinopyrum intermedium. Out of these primers, one could amplify a specific DNA fragment in the accession SZ of Th. intermedium. This fragment, OPF03 1291 , was cloned and sequenced. Sequence data searching in GenBank showed that this fragment had a homologous degree of 88% to a RAPD marker OPF03 1296 (GenBank accession U43516) in Th. bessarabicum. Based on the sequence of OPF03 1291 , two pairs SCAR primers were designed. With OPF03 and primers P3 and P4, RAPD and SCAR analyses were performed on T.aestivum, T.aestivum Th.intermedium partial amphidiploid, Th.elongatum, Th.intermedium, wheat Th.elongatum substitution and addition lines. The results of PCR amplification showed that the RAPD marker was not appeared in materials with genome E e, whereas SCAR marker was appeared in all E genome possessed materials. The SCAR marker derived from the E b genome specific RAPD fragment could be used in the meantime to detect the chromosome of E e genome under common wheat background. |
| Key words: Thinopyrum spp.,genome,RAPD,SCAR |
