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黄萎病菌毒素诱导棉花愈伤组织中POD,SOD活性和PR蛋白的变化
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摘要:
对不同抗、感病品种棉花愈伤组织在黄萎病菌毒素诱导下,体内过氧化物酶和SOD活性的变化进行了测定,结果表明,感病品种中POD活性的升高大且早于抗病品种;感病品种SOD活性随诱导时间延长而迅速下降,耐、抗病品种SOD活性的降低较慢;随着病菌毒素诱导时间的增加,在电泳凝胶透射扫描系统中愈伤组织中有数种病原相关蛋白(PR蛋白)表达。
关键词:  黄萎病菌 棉花愈伤组织 过氧化物酶 病原相关蛋白
DOI:
修订日期:1999-09-21
基金项目:
Verticillium dahliae Toxin Induced Changes in the Activities of Peroxidase and SOD and in the Experession of PR Proteins in Cotton Callus
Abstract:
The callus of cotton varieties with different resistance to cotton verticillium wilt pathogen were inoculated with VD toxin of Verticillium dahliae. The activities of peroxidase (POD) and SOD in them were examined before inoculated and at different days after inoculation. The results showed that the increase of POD activity was earlier and higher in susceptible varieties than in resistant ones. The decrease of SOD activity was greater in susceptible varieties than that in resistant ones. Pathogenesis related proteins(PR proteins) were induced in the callus after inoculation.
Key words:  Verticillium dahliae,callus of cotton,POD activities,SOD,PR proteins