| 摘要: |
| 胚胎玻璃化冷冻保存最重要的条件是胚胎细胞膜对抗冻保护的通透速度，即细胞内外液保护剂达到平衡和时间。温度及抗冻剂的种类不同，其通透性速度各异。本试验将玻璃化抗冻液中常用的几种抗冻保护剂，如乙二醇，丙三醇，二甲基亚砜，乙酰胺及丙二醇的通透性进行比较。 |
| 关键词: 通透性 抗冻保护剂 玻璃化 扩张囊胚 鼠 |
| DOI： |
|
| 基金项目:中国博士后科学基金 |
|
| Permeability Measurement of Expanded Mouse Blastocysts to Various Cryoprotectants |
|
|
|
| Abstract: |
| The speed of a cryoprotectant permeates through the embryonic cells, i. e. the time needed by the concentration of a cryoprotectant in intra-and extra-cellular fluid reaching to equilibration, is the most important requirement condition which affect the cryoprotection of embryos by vitrification. Cryoprotectants are differed the permeability, and the permeability is also different under various temperature conditions. In the experiment, the comparison of permeability of expanded mouse blastocyts to various cryoprotectants used in vitrification commonly, such as ethylene glycol (EG), propylene glycol (PG), dimethyl sulfoxide (DMSO), acetamide (AA) and glycerol (GL) were determined. |
| Key words: permeability,cryoprotectant,vitrification,expanded blastocyst,mouse |
