| 摘要: |
| 从豌豆幼苗及开花植株各器官提取总ＲＮＡ，以豌豆肌动蛋白基因为探针，鉴定肌动蛋白基因在不同器官的表达。ｎＯＲＴＨＥＲＮ和Ｄｏｔｈｌｏｔ结果表明，肌动蛋白基因在豌豆中的表达具有发育阶段特异性，它倾向于在豌豆５天苗的芽和２天苗的根中苗的根中异表达。， |
| 关键词: 肌动蛋白 基因 豌豆 幼苗 器官特异性表达 |
| DOI： |
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| 基金项目:国家自然科学基金!39370162 |
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| Developmental-Stage and Organ-Specific Expression of Actin Genes in Seedlings of Pisum satium |
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| Abstract: |
| Total RNAs were isolated from various organs of pea(Pisum satium) seedlings and flowering plants ) then the actin gene of pea(PEAC1) was used as probe to character-ize the expression of actin gene in different organs. Results of Northern and Dot blot anal-ysis indicated that the expression of actin gene in pea has shown developmental-stage specificity. It tends to express specifically in shoots of 5 day old seedlings and roots of 2 day old seedings of pea. |
| Key words: actin,actin gene,pea seedling,organ-specific expression |
