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薄层培养菊苣花梗诱导花芽分化早期超微结构的变化
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摘要:
菊苣花梗薄层培养花芽分化中细胞超微结构的观察表明,在花芽原基的形成中细胞内线粒体、、高尔基体、内质网、核糖体大量增加,且始终可见线粒体的分裂增殖,大量增加的线粒体为内质网、高尔基体的活动提供能量,内质网和高尔基体为花芽分化合成新的结构蛋白和新的功能蛋白。此外,壁旁体多处可见,且常与内质网、高尔基体相邻分布,其功能可能与物质运输有关。
关键词:  菊苣 薄层培养 花芽分化 超微结构
DOI:
基金项目:高等学校博士学科点专项基金
Ultrastructural Study of Cells During Floral Bud Differentiation of Thin Cell Layer Culture of Cichorium intybus
Abstract:
The ultrastructural research of floral bud differentiation in thin cell layer cul-ture of flower stalk of Cichorium intybus revealed that the mitochondria, Golgi bodies,rough endoplasmic reticulum (RER) increased significantly during the floral bud forma-tion. The mitochondria which were dividing into two were observed in various periods of floral buds differentiation. It was probable that the increased mitochondria supplied ener-gies for the RER and Golgi bodies to synthesize the structural and functional proteins for floral bud differentiation. The results indicated that the process of floral buds differentia-tion was associated with intense metabolism. In addition, the paramural bodies were locat-ed near the RER and Golgi bodies, it may be participate the substance transports.
Key words:  Cichorium intybus,thin cell layer culture,floral bud differentiation,ultrastructure