|Abstract:Slightly acidic electrolyzed water (SAEW) has the advantages of possessing broad-spectrum antimicrobial activity, and effective disinfectant method for the drinking water system quality of layer hen houses. However, some microorganisms can play a role in maintaining intestinal immune homeostasis, prevention of inflammation and other beneficial effects. The objective of this paper was to study on the effects of intestinal microflora of poultry supplemented with slightly acidic electrolyzed water, we study on effects of drinking two different forms of water, slightly acidic electrolyzed water and acidifier water for laying hens. Results showed that: drinking 0.3mg/L slightly acidic electrolyzed water and 0.1mg/L acidifier water, the normal feces rate of birds was improved 10%, pH value and the number of Escherichia coli was decreased , lactic acid bacteria was raised of poultry duodenum, ileum and jejunum . Slightly acidic electrolyzed water can not only by killing aquatic microorganisms, improving water sanitation index, but also can improve the intestinal environment, inhibit the growth of Escherichia coli and improve the superior strains status of lactic acid bacteria by reducing the intestinal pH value.