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棉花耐盐性鉴定方法探讨
苏莹,郭安慧,华金平
中国农业大学 农学院/作物遗传改良北京市重点实验室/杂种优势利用教育部重点实验室, 北京 100193
摘要:
为明确快捷简便、经济有效和稳定可靠的棉花耐盐鉴定方法,收集整理1990—2021年国内外关于棉花耐盐性鉴定的相关文献报道,比较了棉花发育不同时期耐盐性鉴定方法,通过比较、综合已有研究结果提出,室内条件下棉花种子萌发期,采用滤纸发芽法以150 mmol/L NaCl溶液胁迫,调查种子发芽率指标,可作为棉花耐盐性快速鉴定的首选方法;室外条件下棉花苗期耐盐性鉴定推荐土壤含盐量0.40%的盐池处理棉花三叶期苗期,以成活苗率作为筛选标准。
关键词:  棉花  耐盐性  萌发期  幼苗期  鉴定方法
DOI:10.11841/j.issn.1007-4333.2021.12.02
分类号:
基金项目:国家重点研发计划(2018YFD0100303);国家自然科学基金(31530053)
Strategies for evaluation the salt tolerance in cotton
SU Ying,GUO Anhui,HUA Jinping
College of Agriculture and Biotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis andUtilization of Ministry of Education, China Agricultural University, Beijing 100193, China
Abstract:
To establish a unified evaluation method, which is prerequisite for salt tolerance mechanism research, reports on the evaluation strategies in cotton salt tolerance since 1990 were summarized. Meanwhile, the evaluation protocols and corresponding results related to cotton salt tolerance during seed germination, seedling stage and whole growth stages were analyzed. The results showed that the unified evaluation protocols of salt-tolerance in upland cotton are: Under laboratory conditions, the cotton seeds germinating ratio is conducted by using the folding filter paper and keeping it wet at room temperature after treatment with 150 mmol/L NaCl solution at seed germination stage; Under outdoor conditions, the cotton seedlings survival ratio was determined after treatment with 0. 40% NaCl in salt pool at three leaves seedling stage.
Key words:  cotton  salt tolerance  germination stage  seedling stage  evaluation protocol
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