利用小RNA深度测序快速鉴定草莓病毒
投稿时间:2020-11-11    点此下载全文
引用本文:何成勇,高德航,范灵姣,邢飞,李世访,王红清.利用小RNA深度测序快速鉴定草莓病毒[J].中国农业大学学报,2021,26(8):54-60
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作者单位E-mail
何成勇 中国农业大学 园艺学院, 北京 100193
中国农业科学院 植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193 
hchyong2019@163.com 
高德航 中国农业大学 园艺学院, 北京 100193  
范灵姣 中国农业大学 园艺学院, 北京 100193  
邢飞 中国农业科学院 植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193  
李世访 中国农业科学院 植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193  
王红清 中国农业大学 园艺学院, 北京 100193 wanghq@cau.edu.cn 
基金项目:国家重点研发计划(2019YFD1001800);国家重点研发计划政府间重点项目(2017YFE0010900)
中文摘要:病毒侵染直接影响草莓的生长发育及果实品质,快速检测及鉴定病毒是病毒防治的前提。本研究以‘丰香’草莓和‘哈尼’草莓为试材,利用小RNA(sRNA)测序结合RT-PCR技术对2种栽培草莓品种中存在的病毒进行了研究。对sRNA测序结果进行组装和注释,结果表明:在混合样品中共有242个contigs比对到5种草莓病毒,分别为草莓白化病毒(strawberry pallidosis associated virus,SPaV)、草莓镶脉病毒(strawberry vein banding virus,SVBV)、草莓斑驳病毒(strawberry mottle virus,SMoV)、草莓毛形病毒3(strawberry crinivirus 3,SCrV 3)和草莓毛形病毒4(strawberry crinivirus 4,SCrV 4)。利用RT-PCR技术对sRNA测序结果进行验证,结果表明:SMoV、SVBV和SCrV 3在‘丰香’草莓和‘哈尼’草莓中均检测到,而SPaV和SCrV 4只在‘哈尼’草莓样品中检测到。本研究利用sRNA测序技术鉴定了2个草莓品种中存在的病毒,首次在我国生产的同一个草莓品种中检测到5种病毒,为草莓病毒的检测和防控提供了新的思路。
中文关键词:草莓  sRNA测序  草莓白化病毒  草莓毛形病毒  RT-PCR
 
Rapid identification of strawberry viruses by small RNA deep sequencing
Abstract:Virus infection directly affects the growth and fruit quality of strawberry. Rapid detection and identification of virus is the premise of virus control. In order to identify the viruses in cultivated strawberry varieties, the viruses in two strawberry cultivars were studied by small RNA deep sequencing and RT-PCR. ‘Toyonoka' and ‘Honeoye' cultivars were taken as test materials. The small RNA sequencing results were assembled and annotated. It was found that, in the mixture samples, a total of 242 contigs were mapped to five strawberry viruses, namely strawberry vein banding virus(SVBV), strawberry mottle virus(SMoV), strawberry pallidosis associated virus(SPaV), strawberry crinivirus 3(SCrV 3)and strawberry crinivirus 4(SCrV 4). RT-PCR results indicated that SMoV, SVBV and SCrV 3 were detected in ‘Toyonoka' and ‘Honeoye' cultivars, while SPaV and SCrV 4 were only detected in ‘Honeoye'. In this study, the viruses in two strawberry varieties were identified by small RNA deep sequencing. The presence of five viruses in strawberry cultivar was first detected, which provided a new idea for the detection and control of strawberry viruses.
keywords:strawberry  sRNA sequencing  strawberry pallidosis associated virus  strawberry crinivirus  RT-PCR
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