【打印本页】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 1346次   下载 1190 本文二维码信息
码上扫一扫!
分享到: 微信 更多
意大利蜜蜂同日龄不同职能工蜂上颚腺的转录组学分析
高艳1,3,杨振慧1,李秋方1,朱雅楠1,梁立强1,苏松坤1*,聂红毅1,2*
1.福建农林大学 动物科学学院(蜂学学院), 福州 350002;2.福建农林大学 基因组与生物技术研究中心, 福州 350002;3.江西省养蜂研究所, 南昌 330052
摘要:
为揭示意大利蜜蜂哺育蜂上颚腺中蜂王浆合成和分泌相关的分子机制,通过组建人工蜂群收集3日龄工蜂(3 d)、10日龄哺育蜂(10 dN)、10日龄采集蜂(10 dF)、21日龄哺育蜂(21 dN)和21日龄采集蜂(21 dF),利用高效液相色谱技术检测5种不同职能工蜂上颚腺中10-羟基-2-癸烯酸(10-hydroxy-2(E)-decenoic acid,10-HDA)的含量,并在转录组学水平全面分析上颚腺中调控蜂王浆合成和分泌密切相关的基因。结果表明:工蜂上颚腺中 10-HDA 含量随日龄的增加呈逐渐增加趋势,其中21 dF和21 dN上颚腺中10-HDA的含量均显著高于3 d(P<0.05),但同日龄采集蜂上颚腺中10-HDA的含量高于相同日龄哺育蜂;RNA-seq分析发现,46个差异基因在10 dN 上颚腺中的表达量显著高于3 d、10 dF和21 dF,同时在21 dN上颚腺中的表达量也显著高于21 dF;GO和KEGG富集分析显示这些差异基因主要富集在脂肪酸代谢、能量代谢等方面,暗示哺育蜂的上颚腺可能通过增加脂肪酸代谢和能量代谢等促进蜂王浆中相关脂肪酸的合成。综上,在排除日龄影响下,本研究测定了不同职能工蜂上颚腺中10-HDA的变化趋势,同时筛选了46个与哺育蜂上颚腺中蜂王浆合成和分泌密切相关的基因,为深入阐明其分子机制提供基础,也为研究膜翅目昆虫上颚腺的功能提供新视角。
关键词:  意大利蜜蜂  哺育蜂  上颚腺  蜂王浆合成和分泌  10-HDA  相同日龄
DOI:10.11841/j.issn.1007-4333.2021.04.09
分类号:
基金项目:国家现代农业产业技术体系(蜜蜂)项目(CARS-44-KXJ4);国家自然科学基金面上项目(31772684);福建省自然科学基金项目(2018 J05043);福建省省属高校科研项目(JK2017014)
Transcriptome analysis of mandibular glands from worker bees (Apis mellifera ligustica) with different functions at the same age
GAO Yan1,3,YANG Zhenhui1,LI Qiufang1,ZHU Yanan1,LIANG Liqiang1,SU Songkun1*,NIE Hongyi1,2*
1.College of Animal Sciences(College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.Center for Genomics and Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;3.Jiangxi Apiculture Research Institute, Nanchang 330052, China
Abstract:
In order to reveal the molecular mechanism related to the synthesis and secretion of royal jelly in the mandibular glands(MGs)of nurses, specimens(3-day-old worker bees(3 d), 10-day-old nurses(10 dN), 10-day-old foragers(10 dF), 21-day-old nurses(21 dN)and 21-day-old foragers(21 dF))were collected from an established bee colony. The content of 10-hydroxy-2(E)-decenoic acid(10-HDA)was detected in the MGs of worker bees with five different functions by high-performance liquid chromatography(HPLC)and genes closely related to the synthesis and secretion of royal jelly were analyzed comprehensively in the MGs at the transcriptional level. The results showed that: The content of 10-HDA in the MGs of worker bees increased gradually with the increase of age. The content of 10-HDA in the MGs of 21 dN/F were both significantly higher than that of 3 d(P<0. 05), but 10-HDA content of foragers was higher than that of nurses with corresponding age; RNA-seq revealed that the expression of 46 differentially expressed genes(DEGs)in the MGs of 10 dN was significantly higher than those of 3 d, 10 dF and 21 dF, and also those DEGs in the MGs of 21 dN was significantly higher than that of 21 dF; GO and KEGG enrichment analysis showed that up-regulated DEGs were mainly enriched in fatty acid metabolism and energy metabolism, suggesting that the MGs of nurses might increase fatty acid metabolism and energy metabolism to stimulate the synthesis of related fatty acids during the production of royal jelly. In summary, the variation trend of 10-HDA in MGs of worker bees with different performances was determined without the influence of age, and 46 DEGs associated with the secretion of royal jelly were identified, which provide a great deal of valuable information for further elucidating the molecular mechanism underlying the production of royal jelly, and also will provide a new perspective for studying the function of MGs in hymenoptera insects.
Key words:  Apis mellifera ligustica  nurses  mandibular glands  synthesis and secretion of royal jelly  10-HDA  same age
引用本文: