番鸭产蛋前期和产蛋期肝脏组织非靶向代谢组学比较分析
投稿时间:2020-03-28    点此下载全文
引用本文:朱文俊,杨万里,韦聪聪,刘乐,耿照玉,陈兴勇.番鸭产蛋前期和产蛋期肝脏组织非靶向代谢组学比较分析[J].中国农业大学学报,2020,25(12):76-85
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作者单位E-mail
朱文俊 安徽农业大学 动物科技学院, 合肥 230036 2650656928@qq.com 
杨万里 安徽农业大学 动物科技学院, 合肥 230036  
韦聪聪 安徽农业大学 动物科技学院, 合肥 230036  
刘乐 安徽农业大学 动物科技学院, 合肥 230036  
耿照玉 安徽农业大学 动物科技学院, 合肥 230036
安徽农业大学 地方畜禽遗传资源保护与生物育种安徽省重点实验室, 合肥 230036 
 
陈兴勇 安徽农业大学 动物科技学院, 合肥 230036
安徽农业大学 地方畜禽遗传资源保护与生物育种安徽省重点实验室, 合肥 230036 
chenxingyong@ahau.edu.cn 
基金项目:安徽省科技重大专项(201903a06020018);省级创新训练项目(201910364050)
中文摘要:旨在从非靶向代谢组学角度分析番鸭产蛋前期和产蛋期肝脏代谢物谱的差异,选用开产前22周(TT组)和产蛋期40周(FT组)番鸭各12羽作为研究对象,采用超高效液相色谱-电喷雾质谱(UPLC-MS)非靶向代谢组学技术获得24个产蛋前后肝脏代谢物图谱,根据变量权重值(VIP)和独立样本T检验筛选出差异代谢物并作通路富集分析。结果表明,与TT组相比,在FT组筛选出的324种差异代谢物中,156种代谢物丰度均显著上调(P<0.05),168种代谢物丰度均显著下调(P<0.05);脂类及类脂分子和有机酸及其衍生物这2类代谢物数量在上调差异代谢物中分别占19.87%和21.79%,在下调差异代谢物中分别占41.07%和13.1%;通路富集获得17个代谢通路且均显著差异(P<0.05),主要为氨基酸代谢、甘油磷脂代谢、核苷酸代谢和维生素代谢等,其中9个通路为氨基酸代谢通路。除叶酸一碳库、半胱氨酸与蛋氨酸代谢、嘌呤代谢、赖氨酸降解和谷胱甘肽代谢通路下调外,其他12个通路均显著上调(P<0.05),谷胱甘肽和半胱氨酸丰度显著下调(P<0.05),胆碱丰度显著上调(P<0.05)。综上,非靶向代谢组学分析揭示,产蛋期肝脏中脂类及类脂分子和有机酸及其衍生物等高度富集;氨基酸、嘧啶、氨基糖和核苷糖等代谢增加为卵黄前体物合成提供原料。
中文关键词:番鸭  非靶向代谢组学  肝脏  卵黄前体物
 
Comparative analysis of nontargeted metabolomics in liver tissue of Muscovy duck in pre-laying and laying stages
Abstract:In order to analyze the differences of liver metabolite profiles in Muscovy duck during pre-laying and laying stages from the perspective of nontargeted metabolomics, twelve Muscovy ducks were selected at the age of 22 weeks of pre-laying(TT group)and 40 weeks of laying(FT group), respectively. Twenty-four metabolite profiles were obtained by ultra-high performance liquid chromatography-electrospray mass spectrometry(UPLC-MS)nontargeted metabolomics. Differential metabolites were screened out based on variable importance in projection(VIP)and independent-sample T-test and analyzed for pathway enrichment. The results showed that: Compared with TT group, 156 of the 324 differential metabolites in FT group were significantly up-regulated(P<0. 05), and 168 metabolites were significantly down-regulated(P<0. 05). The number of lipids and lipid-like molecules and organic acids and derivatives accounted for 19. 87% and 21. 79% in the up-regulated differential metabolites, and 41. 07% and 13. 1% in the down-regulated differential metabolites, respectively. Seventeen metabolic pathways were obtained by pathway enrichment and showed significant difference(P<0. 05), including amino acid metabolism, glycerophospholipid metabolism, nucleotide metabolism, and vitamin metabolism, among which 9 pathways were amino acid metabolism. In addition to one carbon pool by folate, cysteine and methionine metabolism, purine metabolism, lysine degradation and glutathione metabolism, the other 12 pathways were significantly up-regulated(P<0. 05). The glutathione and cysteine abundance were significantly down-regulated(P<0. 05)and the choline abundance was significantly up-regulated(P<0. 05). In conclusion, the nontargeted metabolomics analysis revealed that lipids and lipid molecules, organic acids and derivatives were highly enriched in the liver during egg-laying period, and the increased metabolism of amino acids, pyrimidine, amino sugar and nucleotide sugar provided essential materials for the synthesis of yolk precursor.
keywords:Muscovy duck  nontargeted metabolomics  liver  yolk precursor
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