干酪乳杆菌对雏鸡十二指肠发育的组织学影响
投稿时间:2019-07-22    点此下载全文
引用本文:王誉颖,刘雪连,卢斯婷,闫雪,韩德平,马云飞.干酪乳杆菌对雏鸡十二指肠发育的组织学影响[J].中国农业大学学报,2020,25(2):61-66
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作者单位E-mail
王誉颖 中国农业大学 动物医学院, 北京 100193 yuyingwang96@163.com 
刘雪连 北京大北农科技集团股份有限公司 饲用微生物工程国家重点实验室, 北京 100192  
卢斯婷 中国农业大学 动物医学院, 北京 100193  
闫雪 北京大北农科技集团股份有限公司 饲用微生物工程国家重点实验室, 北京 100192  
韩德平 中国农业大学 动物医学院, 北京 100193  
马云飞 中国农业大学 动物医学院, 北京 100193 yunfeima@cau.edu.cn 
基金项目:国家重点研发计划项目(2018YFD0500606);国家自然科学基金项目(31772686;31502025)
中文摘要:为探究干酪乳杆菌对雏鸡十二指肠黏膜组织结构和功能及发育的影响,采用组织化学和免疫组织化学方法,以450 只1 日龄SPF健康雏鸡为试验对象,随机分成6 个组,每组5 个重复,每个重复15 只雏鸡。结果表明:1)干酪乳杆菌预防组、治疗组及预防治疗组的肠绒毛高度分别比沙门氏菌攻毒组显著增高了19.04%、10.68%和12.21%;2)与鸡白痢沙门氏菌攻毒组相比,干酪乳杆菌预防组的绒毛高度与隐窝深度的比值(V/C)显著升高23.94%;3)干酪乳杆菌预防组、治疗组的增殖细胞核抗原(PCNA)含量显著高于沙门氏菌攻毒组;4)干酪乳杆菌预防组、治疗组、预防治疗组杯状细胞数量分别比沙门氏菌攻毒组显著升高72.26%、67.74%、65.32%。综上,饲喂干酪乳杆菌进行预防能够显著提高雏鸡十二指肠绒毛高度、V/C值、PCNA含量及杯状细胞数量,提示干酪乳杆菌能有效维护雏鸡肠道健康,减轻鸡白痢沙门氏菌对肠道黏膜组织结构的损害,促进肠道发育。
中文关键词:雏鸡  干酪乳杆菌  鸡白痢沙门氏菌  十二指肠  组织形态
 
Histological effects of Lactobacillus casei on duodenum development in chicksWANG Yuying1, LIU Xuelian2, LU Siting1, YAN Xue2, Han Deping1, MA Yunfei1*
Abstract:To explore the effects of Lactobacillus casei on the structure, function and development of duodenal mucosa in chicks, 450 SPF healthy chicks just hatched were randomly divided into 6 groups. Each group had 5 replicates with 15 chicks in each. Histochemical and immunohistochemical methods were adopted in this study. The results showed that: 1)The intestinal villus height of the L. casei prevention group, the treatment group and the preventive treatment group were significantly higher than that of the Salmonella challenge group by 19. 04%, 10. 68%, and 12. 21%, respectively; 2)The ratio of the villus height to the crypt depth(V/C)of the L. casei prevention group was significantly increased by 23. 94% compared with the Salmonella challenge group; 3)The proliferating cell nuclear antigen(PCNA)content of the L. casei prevention group and the treatment group was significantly higher than that of the Salmonella challenge group; 4)The number of goblet cells in the Lactobacillus prevention, treatment and prevention plus treatment groups was significantly higher than that of the Salmonella challenge group by 72. 26%, 67. 74% and 65. 32%, respectively. The results indicated that L. casei significantly increased the duodenal height, V/C value, PCNA content and goblet cell number of chicks suggesting that L. casei could effectively maintain the intestinal health of chicks and reduce the damage on intestinal mucosal tissue structure caused by Salmonella and promote intestinal development.
keywords:chick  Lactobacillus casei  Salmonella pullorum  duodenum  mucosal morphology
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