| 摘要: |
| 为研究乙酰乙酸（Acetoacetic acid，AcAc）对奶牛中性粒细胞炎症信号通路的影响，以脂多糖（Lipopolysaccharide，LPS）诱导的奶牛中性粒细胞炎症模型为研究对象，利用qRT-PCR、生化和酶联免疫吸附法（ELISA）测定IL-1β、IL-6、TNF-α和IKKβ激酶活性。结果表明：1） qRT-PCR结果显示，与对照组相比较，LPS处理组IL-1β、IL-6和TNF-α mRNA的表达显著增强（P<0.01）。与LPS处理组比较，LPS+AcAc混合处理组，其表达量显著下调（P<0.05）；2）生化检测结果显示，与对照组相比较，LPS处理组中性粒细胞IKKβ激酶活性显著增强（P<0.01）。与LPS处理组比较，LPS+AcAc混合处理组IKKβ激酶活性则显著降低（P<0.05）；3） ELISA结果显示，与对照组相比较，LPS增加促炎因子TNF-α、IL-6和IL-1β的释放。相对于LPS处理组，LPS+AcAc混合处理组促炎因子的释放则显著降低（P<0.01）。综上，AcAc可以抑制LPS诱导的奶牛中性粒细胞炎症信号通路的激活，具有一定的抗炎功能。 |
| 关键词: 奶牛 中性粒细胞 酮体 炎症 先天免疫 |
| DOI：10.11841/j.issn.1007-4333.2019.08.10 |
| 分类号: |
| 基金项目:青年科学基金项目（31602121） |
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| Acetoacetic acid inhibits the activation of inflammatory signal pathway in lipopolysaccharide-stimulated cow neutrophils |
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YU Chunwei1, ZHANG Yuming2, DENG Qinghua2
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1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Animal Science and Technology, Inner Mongolia University for Nationlities, Tongliao 028000, China
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| Abstract: |
| To study the effect of acetoacetic acid (AcAc) on neutrophil inflammatory signaling pathway in dairy cows,a cow neutrophil inflammation model induced by lipopolysaccharide (LPS) was used as the research object.qRT-PCR,biochemistry and enzyme-linked immunosorbent assay (ELISA) methods were adopted in this study.The results showed that:1) Compared with the control group,the qRT-PCR results showed that the mRNA expression levels of IL-1 β,IL-6 and TNF-α were significantly increased in LPS treatment group (P<0.01),while their expression levels were significantly decreased in LPS + AcAc treated group than those in LPS treated group (P<0.05);2) Compared with the control group,the biochemical test results showed that the activity of IKK β kinase was significantly increased in LPS treated group (P<0.01),Its activity was significantly decreased in LPS+AcAc treated group than that in LPS treated group (P<0.05);3) Compared with the control group,the ELISA results showed that LPS increased the release of pro-inflammatory factors TNF-α,IL-6 and IL-1 β.These pro-inflammatory factors were significantly decreased in LPS+AcAc treated group than that in LPS treated group (P<0.01).The results suggested that AcAc inhibited the activation of inflammation signaling pathway in lipopolysaccharide-stimulated cow neutrophils and played anti-inflammatory function. |
| Key words: cow neutrophils ketosis inflammation innate immune |
