酸浆叶茶对四氧嘧啶引起小鼠糖尿病的降血糖作用

Hypoglycemic effect of physalins leaf tea on diabetic mice induced by alloxan

关键词:  小鼠  糖尿病  酸浆叶茶  降糖作用  抗氧化作用

Key words:  mice  diabetes  physalins leaf tea  hypoglycemic effect  antioxidation

摘要:

为研究酸浆叶茶对患糖尿病小鼠的降血糖作用及相关保护作用，选取75只雄性昆明小鼠，随机分成5组，包括模型、酸浆叶茶低、中、高剂量和阳性对照（阿卡波糖）等组。利用四氧嘧啶诱发小鼠糖尿病后，测定小鼠体重、空腹血糖（Fasting blood-glucose，FBG），血清胰岛素（Serum insulin，INS）、谷丙转氨酶（Alanine aminotransferase，ALT）和肌酐（Creatinine，Cr），并测定肝、肾组织的超氧化物歧化酶（Superoxidase dismutase，SOD）、丙二醛（Malondialdehyde，MDA）和谷胱甘肽过氧物酶（Glutathione peroxidase，GSH-PX），小鼠免疫器官指数。结果表明：酸浆叶茶能降低糖尿病小鼠的FBG指标；中、高剂量组能显著促进糖尿病小鼠体重的增长；恢复INS含量；降低ALT和Cr以及肝、肾中MDA含量；提高SOD和GSH-PX活性，能一定程度提高糖尿病小鼠的免疫功能。因此，酸浆叶茶可降低四氧嘧啶引起的小鼠血糖升高，其作用机制可能与改善胰岛素分泌功能和提高机体的抗氧化能力有关。

Abstract:

To investigate the effect of physalins leaf tea on diabetic mice,a total of 75 male Kunming mice were randomly divided into five groups,which were model group,low-dose physalins leaf tea treatment group (0.5 g/kg),middle-dose group (1.0 g/kg),high-dose group (2.0 g/kg),positive control group with acarbose treatment. Diabetes in mice was induced by alloxan,and self-made physalins leaf tea was administrated through intragastric administration for 14 days.After 14 day treatment,the body weight of mice was measured.The content of FBG,INS,ALT,and Cr in serum,activity of SOD and GSH-Px,and content of MDA in liver and kidney were determined.And the immune organ index of diabetic mice was evaluated.The results showed that:FGB in diabetic mice were reduced by physalins leaf tea;compared with the model group,the body weight and INS content of mice in physalins leaf tea (1.0 and 2.0 g/kg) groups were obviously increased.The ALT and Cr levels in serum were significantly decreased;in the liver and kidney,the MDA content were significantly decreased and the activity of SOD and GSH-PX were significantly increased,the immunity of diabetic mice could be promoted by physalins leaf tea.In conclusion,physalins leaf tea could effectively lower the blood sugar lever of mice induced by alloxan.The potential mechanisms of reducing blood sugar were associated with its improving the insulin secretion and antioxidant ability.