黄体期和卵泡期小尾寒羊KiSS-1与RFRP-3组织表达研究

安雪姣; 潘章源; 赵生国; 李春艳; 田志龙; 狄冉; 刘秋月; 胡文萍; 王翔宇; 张效生; 张金龙; 蔡原; 储明星

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*黄体期和卵泡期小尾寒羊KiSS-1与RFRP-3组织表达研究*
安雪姣^1,2, 潘章源^1,3, 赵生国², 李春艳¹, 田志龙¹, 狄冉¹, 刘秋月¹, 胡文萍¹, 王翔宇¹, 张效生⁴, 张金龙⁴, 蔡原², 储明星¹
1.中国农业科学院北京畜牧兽医研究所/农业部动物遗传育种与繁殖重点实验室, 北京 100193;2.甘肃农业大学动物科学技术学院, 兰州 730070;3.临沂大学农林科学学院, 山东临沂 276005;4.天津市畜牧兽医研究所, 天津 300381

摘要:

为分析KiSS-1和RFRP-3基因在小尾寒羊不同繁殖时期（黄体期和卵泡期）下丘脑、垂体、性腺轴各组织中的表达差异，阐明这2个基因在绵羊发情转换中的表达模式，以常年发情的小尾寒羊为研究对象，采用实时荧光定量PCR技术，对比分析了KiSS-1和RFRP-3基因在黄体期和卵泡期的小尾寒羊下丘脑-垂体-性腺轴各组织中的表达差异。结果表明：1）KiSS-1和RFRP-3基因在下丘脑、垂体、卵巢等10种组织中均有表达；2）黄体期和卵泡期下丘脑中KiSS-1基因表达量均显著高于其他组织（P<0.05），卵泡期下丘脑和松果体中KiSS-1基因表达量均显著高于黄体期（P<0.05）；3）黄体期和卵泡期下丘脑和卵巢中RFRP-3基因的表达量均显著高于其他组织（P<0.05），且卵巢中RFRP-3基因在黄体期的表达量显著高于卵泡期（P<0.05）。综上，研究发现KiSS-1和RFRP-3基因在绵羊发情调控中均发挥重要作用，KiSS-1促进发情而RFRP-3抑制发情。

关键词: 小尾寒羊繁殖时期 KiSS-1 RFRP-3 组织表达

DOI：10.11841/j.issn.1007-4333.2019.02.09

分类号:

基金项目:国家自然科学基金项目（31472078）；国家肉羊产业技术体系专项（CARS-38）；中央级公益性科研院所基本科研业务费专项（Y2017JC24）；中国农业科学院科技创新工程（ASTIP-IAS13）；农业科研杰出人才及其创新团队项目；国家万人计划科技创新领军人才项目

Tissue expression of KiSS-1 and RFRP-3 in Small Tail Han sheep at luteal and follicular periods

AN Xuejiao^1,2, PAN Zhangyuan^1,3, ZHAO Shengguo², LI Chunyan¹, TIAN Zhilong¹, DI Ran¹, LIU Qiuyue¹, HU Wenping¹, WANG Xiangyu¹, ZHANG Xiaosheng⁴, ZHANG Jinlong⁴, CAI Yuan², CHU Mingxing¹

1.Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;3.College of Agricultural and Forestry Science, Linyi University, Linyi 276005, China;4.Tianjin Institute of Animal Sciences, Tianjin 300381, China

Abstract:

The aim of this study was to analyze the expression of KiSS-1 and RFRP-3 genes in the tissues of the hypothalamus-pituitary-gonadal axis in Small Taili Han sheep during different reproductive periods (luteal and follicular periods), and to elucidate the expression pattern of these two genes in estrus conversion of sheep. Small Tail Han ewes at one year-round estrous breed were selected in the study, and Real-time PCR method was used to detect the expression of KiSS-1 and RFRP-3 genes in hypothalamus-pituitary-gonadal axis' tissues during luteal and follicular periods. The results showed that KiSS-1 and RFRP-3 genes were all expressed in ten tissues such as hypothalamus, pituitary and ovary. The expression of KiSS-1 in hypothalamus was significantly higher (P<0.05) than that in other tissues at both luteal and follicular periods. The expression of KiSS-1 in hypothalamus and pineal gland was significantly higher (P<0.05) in follicular period than that in luteal period. The expression of RFRP-3 in hypothalamus and ovary was significantly higher (P<0.05) than that in other tissues both luteal and follicular periods. The expression of RFRP-3 in ovary was significantly higher (P<0.05) in luteal period than that in follicular period. In conclusion, the KiSS-1 and RFRP-3 genes played important roles in sheep estrus regulation. KiSS-1 promoted estrus while RFRP-3 inhibited estrus.

Key words: Small Tail Han sheep reproductive period KiSS-1 RFRP-3 tissue expression

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