乳酸链球菌素及大黄组方对子宫内膜炎大鼠结肠的影响

Effects of nisin and rhubarb compounds on the colon of rats with endometritis

关键词:  大鼠  乳酸链球菌素  大黄组方  子宫内膜炎  结肠  性激素  致病性大肠杆菌

Key words:  rats  nisin  rhubarb compound  endometritis  colon  sex hormone  Escherichia coli

摘要:

为研究乳酸链球菌素（Nisin）及大黄组方对患子宫内膜炎大鼠生殖系统内分泌调控与结肠炎症因子的影响，进一步揭示子宫内膜炎与结肠之间的关系，构建子宫内膜炎模型，建模前后用ELISA试剂盒检测各组大鼠子宫中髓过氧化物酶（MPO）、催产素（OXT）和检测患子宫内膜炎大鼠结肠中白介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）以及结肠菌群的变化情况来确定治疗效果。结果表明：1）造模前后，空白对照组中MPO和OXT的浓度无显著性差异；造模24 h，Nisin组、大黄组方中和高剂量组中MPO的浓度显著降低；大黄组方高剂量组和阳性对照组中OXT的浓度显著高于阴性对照组和空白对照组，阴性对照组显著降低；造模48 h，阴性对照组中OXT的浓度显著降低，且显著低于其他各组；造模72 h，阴性对照组中MPO显著升高，OXT的浓度显著降低，大黄组方高剂量组和阳性对照组可显著降低子宫中MPO的浓度和显著增加OXT的浓度。2）在结肠中，患子宫内膜炎大鼠在0~72 h阶段，阴性对照组中IL-1β和TNF-α的浓度显著升高，大黄组方高剂量组、Nisin组及阳性对照组中IL-1β和TNF-α的浓度显著降低。3）Nisin及大黄组方高剂量组显著增加结肠中乳酸杆菌和双歧杆菌的数量，显著减少大肠杆菌和肠球菌的数量。研究发现Nisin和大黄组方高剂量组可降低子宫内膜炎大鼠子宫中MPO和OXT的浓度，患子宫内膜炎大鼠使结肠中IL-1β和TNF-α的浓度升高，Nisin、大黄组方高剂量组和阳性对照组可降低IL-1β和TNF-α的浓度，还可以调节结肠中菌群结构。

Abstract:

The aim of this study was to investigate the effects of nisin and rhubarb compounds on the endocrine regulation and colon inflammation of rats with endometritis, and to further reveal the relationship between endometritis and the colon. Excluding the blank control, the models in other groups were established by using Escherichia coli. ELISA kit was applied to detect MPO and OXT in rat's uterus. The IL-1 β and TNF-α in the colon of rat with endometritis were detected and the changes of colon flora to determine the therapeutic effect were also investigated. The results showed that:There was no significant difference in concentrations of MPO and OXT between groups at 0 h before modeling. At 24 h after modeling, the concentration of MPO in the middle and high dose of rhubarb compounds groups and nisin group were decreased. The concentration of OXT in the high dose of rhubarb compounds and the positive control group was higher than that in the negative control group and the blank control group, while that was decreased in the negative control group. After 48 hours of modeling, the concentration of OXT in the negative control group was significantly reduced. At 72 hours after the establishment of model, MPO in the negative control group was increased, and the concentration of OXT was decreased. In uterus, the concentration of MPO and OXT in high dose of rhubarb compounds and positive control group were significantly increased. 2) In the colon, the levels of IL-1 β and TNF-α in the negative control group were significantly increased during the 0 to 72 h. The concentrations of IL-1 β and TNF-α in high dose of rhubarb compounds groups, the nisin group, and the positive control group were significantly reduced. 3) In colon, the number of Lactobacillus and Bifidobacterium in high dose of rhubarb compounds groups and the nisin group increased, and the number of Escherichia coli and Enterococcus were decreased. In conclusion, the concentration of MPO and OXT were decreased in rats by applying the nisin and rhubarb compound. In rats with endometritis, the concentration of IL-1 β and TNF-α in the colon were increased. High doses of rhubarb compounds and the nisin group could regulate the structure of colonic microbiota.