| 本文已被：浏览 622次 下载 324次 |
码上扫一扫！ |
|
|
| 中国储粮害虫DNA条形码鉴定系统研究 |
|
吴志刚1, 李文欣1, 赵紫华1, 伍祎2, 张涛2, 曹阳2, 李福君2, 李志红1
|
|
1.中国农业大学 植物保护学院, 北京 100193;2.国家粮食局科学研究院, 北京 100037
|
|
| 摘要: |
| 为一步提升我国储粮害虫快速、准确鉴定能力，采用微软产品技术解决方案，应用SQL SERVER 2008数据库和C#编程语言，研制了中国储粮害虫DNA条形码鉴定系统（Grain Pests DNA Barcode Identification System，GPDBIS）。GPDBIS收集了我国将近40种储粮昆虫及仓储螨类的基础信息和mtDNACOⅠ条形码信息，提供了基于DNA条形码的相似度比对鉴定功能和系统发育树分析鉴定功能、基础信息查询及管理功能。GPDBIS初步应用效果显示，该系统界面友好、功能稳定，能够实现我国常见储粮害虫的快速、准确鉴定。 |
| 关键词: 专家系统 储粮害虫 DNA条形码 相似度比对 系统发育树 |
| DOI：10.11841/j.issn.1007-4333.2017.05.010 |
| 分类号: |
| 基金项目:国家国际科技合作专项项目（2013DFG32350） |
|
| Development and primary application of the DNA barcode identification system of grain pests |
|
WU Zhigang1, LI Wenxin1, ZHAO Zihua1, WU Yi2, ZHANG Tao2, CAO Yang2, LI Fujun2, LI Zhihong1
|
|
1.College of Plant Protection, China Agricultural University, Beijing 100193, China;2.Academy of State Administration of Grain, Beijing 100037, China
|
| Abstract: |
| For rapid and accurate identification of stored-product pests,Grain Pests DNA Barcode Identification System (GPDBIS) was established by using SOL SERVER 2009 and C#.The basic information and mtDNACOⅠ barcode information of about 40 species of stored-product pests and mites were collected in the GPDBIS system.This system provided similarity comparison,phylogenetic tree,information searching and management.The preliminarily application showed that the GPDBIS system was interface friendly and function stably,which achieved rapid and correct identification of stored-product pests.GPDBIS developed in this study could further enhance the ability of rapid and correct identification of stored-product pests,improved the monitoring and management of stored-product pests.It could also facilitate the international trade of agricultural goods and grain safety of China. |
| Key words: expert system stored-product pest DNA barcoding similarity comparison phylogenetic tree |
