| 摘要: |
| 为分析发酵乳对肥胖小鼠肝脂代谢和肠道菌群的影响，本研究将60只C57BL/6小鼠随机分为5组：正常对照组（CON）、模型组（MD）、易善复组（YG）、低浓度发酵乳干预组（LFG）和高浓度发酵乳干预组（HFG）。肥胖模型造模成功后进行为期6周的灌胃，试验结束测量小鼠的空腹体重和身长，计算LEE’S指数，采血检测生化指标，采集肝脏组织观察病理变化；采集小鼠盲肠内容物进行肠道菌群测序。结果表明：1）与CON相比，MD小鼠体重、LEE’S指数、ALT和AST均极显著提升（P<0.01），表明肥胖模型建立成功。2）与MD相比，发酵乳干预组小鼠体重、LEE’S指数、ALT和TC均极显著下降（P<0.01），其中AST和TG显著下降（P<0.05），同时发酵乳和易善复组小鼠肝组织脂肪变性有明显好转，基本没有明显的脂肪空泡。表明发酵乳可以抑制肥胖小鼠体重的增加，改善肥胖小鼠的肝脂代谢功能。3）与MD相比干预组小鼠肠道菌群的多样性水平有一定程度的恢复，尤其是厚壁菌门和拟杆菌门。表明发酵乳对于小鼠肠道菌群多样性的恢复有一定的作用。综上，本研究阐明了发酵乳对肥胖小鼠肝脂代谢和肠道菌群的影响，为功能型发酵乳的开发提供理论依据。 |
| 关键词: 发酵乳 肥胖 肝脂代谢 肠道菌群 |
| DOI：10.11841/j.issn.1007-4333.2024.08.10 |
| 投稿时间：2023-09-13 |
| 基金项目:优才计划学生发展支持项目（XSZZ2021003） |
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| Effects of fermented milk on liver lipid metabolism and intestinal flora in obese mice |
|
LI Haoxia1, ZHANG Daoliang1, XUE Zhilin1, ZHANG Tianlin2, FENG Shibin1, ZHAO Chang1, WANG Xichun1, LI Yu1*
|
| (1.College of Animal Science and Technology, Anhui Agricultural University,Hefei 230031,China;2.College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271000,China) |
| Abstract: |
| To analyze the effects of fermented milk on liver lipid metabolism and intestinal microbiota in obese mice， sixty C57BL/6 mice were randomly divided into five groups： control group （CON）， model group （MD）， Yishanfu group （YG）， low concentration fermented milk intervention group （LFG）， and high concentration fermented milk intervention group （HFG）. After successful modeling of obesity， a 6-week gastric lavage was performed. At the end of the experiment， the weight and length of the mice were measured after fasting， the LEE'S index was calculated， the blood was collected for biochemical indicators and pathological changes in liver tissue were observed， and cecal contents were obtained from mice for gut microbiota sequencing. The results showed that ：1） Compared with CON， the body weight， LEE’S index， ALT， and AST of MD mice were significantly increased （P<0.01）， indicating that the obesity model was successfully established. 2） Compared with MD， the body weight， LEE'S index， ALT， and TC of the fermented milk intervention group mice were significantly reduced （P<0.01）， with AST and TG significantly reduced （P<0.05）. At the same time， the fatty degeneration of liver tissue in the fermented milk and Yishanfu group of mice was significantly improved， with almost no obvious fat vacuoles， indicating that fermented milk can inhibit the weight gain of obese mice， and improve their liver lipid metabolism function.3） Compared with MD， the diversity level of intestinal microbiota in the intervention group of mice showed a certain degree of recovery， especially in the Firmicutes and Bacteroidetes.It indicated that fermented milk had a certain effect on the restoration of intestinal microbiota diversity in mice. In summary， this study clarified that effects of fermented milk on liver lipid metabolism and intestinal flora in obese mice， providing a theoretical basis for the development of functional fermented milk. |
| Key words: fermented milk obesity liver lipid metabolism intestinal microbiota |
