| 摘要: |
| 为探究gga-miR-17-5p在鸡脂肪沉积和肌肉发育中的作用，本研究以矮小品系S3（DW）和隐性白羽鸡（RR）为试验素材，分析gga-miR-17-5p在不同时期腿肌、肝脏、腹脂组织及细胞中的表达差异，并通过KEGG及蛋白互作分析筛选关键靶基因。结果表明：1）gga-miR-17-5p在腹脂、肝脏和腿肌组织中均可表达。在腹脂中，gga-miR-17-5p在0周表达有品种差异（P<0.05），且2个品种在0周时显著高于其他周龄（P<0.05）；在肝脏中，gga-miR-17-5p在16周有品种差异（P<0.05），S3系在16周显著高于其他周龄（P<0.05），隐性白羽肉鸡在16周显著高于0周和8周（P<0.05）；在腿肌中，gga-miR-17-5p在0周有品种差异（P<0.05），S3系在0周和2周显著于其它周龄（P<0.05），隐性白羽肉鸡在0周显著高于其他周龄（P<0.05）。2）在脂肪细胞中，gga-miR-17-5p在肌内脂肪细胞诱导分化6 d后的表达显著高于增殖期和分化1 d（P<0.05）；腹脂细胞分化1 d的表达最低，显著低于增殖期、分化4 d和分化6 d（P<0.05）。3）在成肌细胞中，gga-miR-17-5p的表达量随分化时间的延长而升高，且分化6 d的表达显著高于增殖期（P<0.05）。4）KEGG及蛋白互作分析表明，PTEN、MAPK3、PIK3R1、BECN1和PLCB4是gga-miR-17-5p重要的靶基因。RT-qPCR结果表明，PTEN和PIK3R1在成肌细胞增殖期的表达显著高于分化期（P<0.05）。综上，gga-miR-17-5p的表达存在显著的品种和组织差异性，miR-17-5p很可能通过PTEN、MAPK3、PIK3R1、BECN1和PLCB4来影响肌肉发育和脂肪沉积，其中PTEN和PIK3R1尤为关键。本研究为深入研究gga-miR-17-5p的功能和机制提供了坚实的理论基础和数据支撑。 |
| 关键词: 鸡 gga-miR-17-5p 脂肪沉积 肌肉发育 |
| DOI：10.11841/j.issn.1007-4333.2024.07.03 |
| 投稿时间：2023-12-15 |
| 基金项目:国家重点研发计划“农业生物种质资源挖掘与创新利用”重点专项资助（2021YFD1200803）；现代农业产业技术体系建设专项（CARS-41-Z05）；江苏省种业振兴揭榜挂帅项目（JBGS（2021）109；JBGS［2021］029）；国家自然科学基金项目（32102538）；广东省基础与应用基础研究基金项目（2022A1515012014）；扬州市现代农业项目（YZ2023054） |
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| Differential expression of gga- miR-17-5p in chicken adipose and skeletal muscle tissues and cells and screening of its key target genes |
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YANG Miaomiao1,2, XIANG Hai2, LI Ruirui1,2, ZHAO Zhenhua1, WANG Qianbao1, HUANG ZhengYang1, LI Chunmiao1, LIANG Zhong1, WU Zhaolin1, HUANG Huayun1*
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| (1.Institute of Poultry Science, Yangzhou 225125,China;2.Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding/ School of Life Science and Engineering, FOSHAN University, Foshan 528225,China) |
| Abstract: |
| To investigate the role of gga-miR-17-5p in chicken fat deposition and muscle development， the study used dwarf strain S3 （DW） and recessive white feathered chickens （RR） as test materials to analyze the expression differences of gga-miR-17-5p in bone and adipose tissues and cells at different periods of time ， and to screen the key target genes by KEGG and protein interactions analysis. The results showed that： 1） gga-miR-17-5p was expressed in abdominal fat， liver and leg muscle tissues. In abdominal fat， there was a variety difference in the expression of gga-miR-17-5p at 0 week （P<0.05）， and the two varieties were significantly higher in two breeds at week 0 than other weekly ages （P<0.05）. In liver， gga-miR-17-5p was expressed with breed differences at week 16 （P<0.05）， and was significantly higher in S3 line at week 16 than other weekly ages （P<0.05）， and was significantly higher in cryptic white-feathered broilers at 16 weeks was significantly higher than at 0 and 8 weeks （P<0.05）. In the leg muscle， there was a breed difference in gga-miR-17-5p at 0 weeks （P<0.05）， and the S3 line was significantly higher than other weeks at 0 and 2 weeks （P<0.05）， The recessive white feathered chickens were significantly higher than other weeks of age at 0 weeks （P<0.05）. 2） In adipocytes， the expression of gga-miR-17-5p in intramuscular adipocytes after 6 days of induction of differentiation was significantly higher than that in the proliferation phase and 1 day of differentiation. （P<0.05） The lowest expression was found in abdominal adipocytes at 1 d of differentiation， which was significantly lower than that in the proliferative stage， at 4 d of differentiation， and at 6 d of differentiation（P<0.05）.3） In myoblasts， the expression of gga-miR-17-5p was elevated with the prolongation of the time of differentiation， and it was significantly higher in 6 d of differentiation than that in the proliferative stage （P<0.05）. 4） KEGG and protein interactions analysis showed that PTEN， MAPK3， PIK3R1， BECN1 and PLCB4 were important target genes of gga-miR-17-5p. The RT-qPCR results showed that the expression of PTEN and PIK3R1 was significantly higher in the proliferative stage of myoblasts than that in the differentiation stage （P<0.05）. Taken together， the expression of gga-miR-17-5p exhibits significant species and tissue variability and miR-17-5p is likely to affect muscle development and fat deposition through PTEN， MAPK3， PIK3R1， BECN1 and PLCB4， of which PTEN and PIK3R1 are particularly critical. This study provides a solid theoretical foundation for the in-depth study of gga-miR-17-5p function and mechanism. |
| Key words: Chicken gga-miR-17-5p fat deposition muscle development |
