| 摘要: |
| 为分析清远麻鸡不同组织中miR-142-3p的表达情况，并筛选其在脾脏中的关键靶基因，本研究利用在线数据库预测鸡miR-142-3p靶基因，并对靶基因进行GO和KEGG富集分析以及构建蛋白-蛋白互作网络。利用在线公共数据库，分析脾脏相对于其他组织的miR-142-3p表达量；再根据miRNA表达量与其靶基因表达量负相关机制，对miR-142-3p靶基因与脾脏中相对其他组织显著下调基因取交集，筛选潜在靶基因。结果表明：1）预测获得85个靶基因，GO富集分析显示，靶基因参与多种高分子化合物的合成与转运过程。2）KEGG结果显示富集到MAPK、Notch、Wnt等多条与抗肿瘤免疫相关的信号通路。3）PPI网络共筛选到4个Hub基因。4）miR-142-3p在脾脏中的表达量极显著高于肝脏、肺脏、胸肌、心脏和肾脏（P<0.01）。5）共筛选到TWF1、MYH10、NFIB、FNBP1L和FERMT2 5个靶基因。综上，鸡miR-142-3p在脾脏中很可能通过TWF1、MYH10、NFIB、FNBP1L和FERMT2 发挥生物学功能。本研究为解析鸡miR-142-3p的功能及其分子机制提供了参考和依据。 |
| 关键词: 清远麻鸡 miR-142-3p 生物信息学 组织表达 脾脏 |
| DOI：10.11841/j.issn.1007-4333.2024.07.02 |
| 投稿时间：2023-12-27 |
| 基金项目:国家肉鸡产业技术体系项目（CARS-41-G07）；“十四五”四川省畜禽育种攻关项目（2021YFYZ0031）；四川省区域创新合作项目（2023YFQ0035） |
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| MiR-142-3p analysis of Qingyuan partridge chickens and screening of key target genes |
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ZHU Shiliang, YU Chunlin, QIU Mohan, ZHANG Zengrong, XIONG Xia, HU Chenming, YANG Li, PENG Han, CHEN Jialei, SONG Xiaoyan, XIA Bo, YANG Chaowu*
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| (Animal Breeding and Genetics Key Laboratory，Sichuan Animal Science Academy， Chengdu 610066，China) |
| Abstract: |
| The aim of the study is to analyze the expression of miR-142-3p in different tissues of Qingyuan partridge chickens and screen for its key target genes in spleen. In this study， the online database was used to predict the target genes of chicken miR-142-3p， and the GO， KEGG and protein-protein interaction network analysis of the target genes were performed. The online public database was used to analyze the expression of miR-142-3p in spleen compared with other tissues. Based on the mechanism of negative correlation between miRNA expression and target gene expression， the intersection of miR-142-3p target genes and significantly down-regulated genes in spleen compared to other tissues was taken to screen potential target genes. The results showed that： 1） A total of 85 target genes were obtained， and GO enrichment analysis showed that the target genes were involved in the synthesis and transport of a variety of polymers. 2） KEGG results showed that MAPK， Notch， Wnt and other signaling pathways related to anti-tumor immunity were enriched. 3） A total of 4 Hub genes were screened by PPI network. 4） miR-142-3p expression was significantly higher in spleen than that in liver， lung， pectoral muscle， heart and kidney. 5） Five target genes were screened including TWF1、MYH10、NFIB、FNBP1L and FERMT2. In summary， chicken miR-142-3p is likely to exert biological functions in the spleen through TWF1、MYH10、NFIB、FNBP1L and FERMT2. This study provides a reference and basis for analyzing the function and molecular mechanism of chicken miR-142-3p. |
| Key words: Qingyuan partridge chickens miR-142-3p bioinformatics spleen |
