| 摘要: |
| 为进一步验证SLC38A11在丝羽乌骨鸡黑色素沉积中的作用，本研究采用基于飞行时间质谱分析技术对SLC38A11基因SNPs位点进行基因分型，对所有SNP位点利用Hapoview4.1进行连锁不平衡分析，并检测SNP位点是否处于H-W平衡状态，分析基因型与皮肤亮度L*值关联性，筛选对皮肤乌色度显著相关的SNP标记。结果表明：1）SLC38A11基因4个多态性位点质谱检出率为100%，每个位点都有3种基因型。SLC38A11基因SNP1位点为错义突变，SNP2、SNP3和 SNP4 3个SNPs位点均为同义突变。2）卡方检验表明，SNP1位点偏离H-W平衡（P<0.05），其他3个SNPs位点均处于H-W平衡状态（P>0.05）。SNP1位点遗传多样性较低，其他3个SNPs位点为中度多态。3）单标记关联分析表明，SNP1位点TC基因型的胸部皮肤亮度L*值显著低于CC和TT基因型（P<0.05）；SNP2位点AA基因型的胸部皮肤亮度L*值显著低于GG与GA基因型（P<0.05）；SNP3位点TT基因型的胸部皮肤亮度L*值显著低于CC与CT基因型（P<0.05）。4）SNP2、SNP3和SNP4之间的强连锁产生了3种单倍型，H3H3单倍型（AATTAA基因型）胸部皮肤亮度L*值显著低于单倍型H2H3（GACTAG）和H2H1（GGCCAA）。SNP1～SNP4 4个位点基因型合并后，TCAATTAA胸部皮肤亮度L*值显著低于CCGGCCAA、TTGACTAG和CCGACTAG。综上，SLC38A11基因与丝羽乌骨鸡胸部皮肤乌色度密切相关，TCAATTAA基因型可作为研究丝羽乌骨鸡胸部皮肤乌色度的候选分子标记，为下一步利用分子标记辅助育种加快培育乌色度高的丝羽乌骨鸡新品种提供参考。 |
| 关键词: 乌骨鸡 SLC38A11 乌色度 SNP位点 连锁不平衡 |
| DOI：10.11841/j.issn.1007-4333.2024.07.01 |
| 投稿时间：2023-12-20 |
| 基金项目:江苏省农业自主创新基金项目（CX（21）2011-1）；江苏省种业振兴揭榜挂帅项目（JBGS［2021］107）；现代农业产业技术体系建设专项资金（CARS-41）；扬州市现代农业项目（YZ2021029）；江苏省现代农业产业技术体系（JATS［2023J396］） |
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| Genotyping of SLC38A11 gene in Silky fowls and its association with skin blackness |
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TU Yunjie1, ZHANG Ming1, JU Xiaojun1, JI Gaige1, LIU Yifan1, SHAN Yanju1, ZOU Jianmin1, SHU Jingting1*, ZHAO Weidong2, ZHENG Guoqing2
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| (1.Jiangsu Institute of Poultry Sciences/Key Lab of Poultry Genetics and Breeding of Jiangsu Province/Key Lab for Evaluation and Utilization of Livestock and Poultry Resources (Poultry) of the Ministry of Agriculture and Rural Affairs, Yangzhou 225125,China;2.Taihe Fengsheng Agriculture and Animal Husbandry Technology Co., Ltd., Ji'an 343700,China) |
| Abstract: |
| To further verify the role of SLC38A11 in melanin deposition in black bone chickens， this study genotyped the SNPs loci of the SLC38A11 gene based on time-of-flight mass spectrometry analysis technology. Hapoview 4.1 was used to analyze the linkage disequilibrium of all SNP sites， and detected whether the SNP sites were in an H-W equilibrium state， and analyzed the correlation between genotype and skin brightness L* value， and screened SNP markers that were significantly correlated with skin blackness. The results were as follows：1）The mass spectrometry detection rate of four polymorphic loci in the SLC38A11 gene was 100%， and each locus had three genotypes. The SNP1 of SLC38A11 gene was a missense mutation， the SNP2， SNP3 and SNP4 were all synonymous mutations. 2）Chi-squared test showed that SNP1 deviated from Hardy-Weinberg equilibrium balance（P<0.05） and the other three SNPs were in Hardy-Weinberg equilibrium balance（P>0.05）. The genetic diversity of SNP1 locus was lower， and the other three SNPs loci were moderately polymorphic. 3）Single marker association analysis showed that the breast skin brightness L* value of the TC genotype at the SNP1 locus was significantly lower than that of the CC and TT genotypes（P<0.05）. The breast skin brightness L* value of the AA genotype at the SNP2 locus was significantly lower than that of the GG and GA genotypes （P<0.05）. The breast skin brightness L* value of the TT genotype at the SNP3 locus was significantly lower than that of the CC and CT genotypes（P<0.05）. 4）The Linkage disequilibrium analysis of four SNPs showed that the strong linkage among SNP2， SNP3 and SNP4 produced three Haplotypes. Association analysis found that the breast skin color brightness L* value of H3H3 Haplotype （AATTAA genotype） was significantly lower than that of Haplotype H2H3（GACTAG）and H2H1 （GGCCAA）. After the combination of SNP1-SNP4 genotypes， the breast skin color brightness L* value of TCAATTAA was significantly lower than that of CCGGCCAA， TTGACTAG and CCGACTAG. the TCAATTAA genotype had a lower L* value for breast skin brightness and a higher blackness. In summary， the SLC38A11 gene is closely related to the breast skin blackness， and the TCAATTAA genotype can be used as a candidate molecular marker for studying the breast skin blackness in Silky fowls. It will provide a theoretical reference for using molecular marker assisted breeding to accelerate the cultivation of new Silky fowls with higher blackness. |
| Key words: black-bone chickens SLC38A11 blackness SNP sites linkage disequilibrium |
