| 摘要: |
| 为分析贝莱斯芽孢杆菌与戊糖片球菌联合使用对饲喂霉变饲料肉鸡的生产性能与肠道损伤的影响，本研究选取96只1日龄白羽肉鸡公雏，随机平均分为空白组、攻毒组、益生菌组和解毒组。每组3个重复，每个重复8只鸡，试验周期为21 d。统计并比较分析不同组肉鸡体重、采食量、料重比；利用ELISA检测血清生化指标的变化；应用荧光定量PCR法检测空肠组织紧密连接蛋白和炎症因子mRNA表达水平的变化；应用病理组织学观察各组肉鸡肠道结构的变化。结果表明： 1）与攻毒组相比，解毒组21日龄肉鸡的体重、平均采食量和平均日增重均显著高于攻毒组（P<0.05）。2）与攻毒组相比，解毒组肉鸡的血清SOD活性、GSH-px活性和MDA含量显著降低（P<0.05），T-AOC含量极显著升高（P<0.01），各项血清生化指标均显著回调（P<0.05）。3）与攻毒组相比，解毒组肉鸡空肠绒毛形态显著改善，空肠组织中CIT含量极显著升高（P<0.01），DAO、D-乳酸和IFABP含量均发生极显著降低（P<0.01）。4）与攻毒组相比，解毒组肉鸡空肠紧密连接蛋白相关基因闭锁蛋白1（ZO-1），闭合蛋白（Occludin）和紧密连接蛋白1（Claudin-1）的mRNA表达水平极显著上升（P<0.01）。5）与攻毒组相比，解毒组肉鸡空肠炎症因子白介素6（IL-6）、白介素8（IL-8）和肿瘤坏死因子α（TNF-α）的mRNA表达水平均发生极显著回调（P<0.01），白介素1（IL-1）的mRNA表达水平均发生显著回调（P<0.05）。综上，贝莱斯芽孢杆菌和戊糖片球菌联合使用可改善霉变饲料造成的肉鸡生产性能下降，减轻霉变饲料导致的肉鸡肠道形态学改变、屏障损伤与炎症反应。本研究为生产实践中联合使用这2株益生菌作为饲料添加剂防控畜禽霉菌毒素中毒提供理论和实践基础。 |
| 关键词: 霉菌毒素 益生菌 肉鸡 空肠炎症 肠道屏障 |
| DOI：10.11841/j.issn.1007-4333.2024.04.19 |
| 投稿时间：2023-06-19 |
| 基金项目:国家自然科学基金项目（32273074；31972746；31872538；31772809）；辽宁省教育厅重点项目（LJKZ0632） |
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| Effect of combined use of Bacillus velezensis and Pediococcus pentosaceus on the production performance and intestinal damage of broilers fed with moldy feed |
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LI Jiahui1,2, WANG Shuo1, LONG Miao1*
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| (1.Key Laboratory of Important Livestock Diseases of Ministry of Education/College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China;2.Comprehensive Service Center of Guosong Town, Tonghua County, Tonghua 134103, China) |
| Abstract: |
| To analyze the effects of the combination of Bacillus subtilis and Pediococcus pentosaceus tablets on the performance and intestinal damage of broilers fed moldy feed， 96 1-day-old white feather broiler male chicks were randomly divided into a blank group， an attack group， a probiotic group and a detoxification group. Each group had 3 replicates， each with 8 chicks， and the test period was 21 d. The changes in body weight， feed intake and feed-to-weight ratio of broilers in different groups were counted and compared. Changes in serum biochemical indexes were detected by ELISA， and changes in the expression levels of proteins of the jejunum and inflammatory factor mRNA were detected by fluorescence PCR， and changes in the intestinal structure of broilers were observed by histopathology. The results showed that： 1） Compared with the attack group， the body weight， average feed intake， and average daily weight gain of 21-day-old broilers in the detoxification group were significantly higher （P<0.05）. 2） Compared with the attack group， the serum SOD activity， GSH-px activity and MDA content of broilers in the detoxification group were significantly decreased （P<0.05）， the T-AOC content was highly significantly increased （P<0.01）， and all the serum life indexes were significantly regressed （P<0.05）. 3） Compared with the attack group， the morphology of jejunal villi of broilers in the detoxification group was significantly improved， the citrulline （CIT） content in jejunal tissues was highly significantly elevated （P<0.01）， and highly significant reductions in the contents of diamineoxidase （DAO）， D-lactic acid and intestinal fatty acid binding protein （IFABP） occurred （P<0.01）. 4） The mRNA expression levels of the jejunal tight junction protein-related genes occludin 1 （ZO-1）， occludin and claudin-1 were highly significantly increased in the detoxification group compared with the attack group （P<0.01）. 5） Compared with the attack group， the mRNA expression levels of the broiler jejunal inflammatory factors IL-6， IL-8 and TNF-α were all highly significantly （P<0.01） retuned in the detoxification group， and the mRNA expression level of IL-1β， was significantly （P<0.05） retuned in the detoxification group. In conclusion， the combined use of Bacillus subtilis and Pediococcus pentosaceus can improve the decline in broiler performance caused by moldy feed， reduce the morphological changes， barrier damage and inflammatory response of the intestinal tract of broilers caused by moldy feed. This study can provide a theoretical and practical basis for the combined use of these two probiotics as feed additives in production practice to prevent and control mycotoxin poisoning. |
| Key words: mycotoxins probiotics broilers jejuno-intestinal inflammation intestinal barrier |
