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| 鸡RUNX3基因组织表达及生物信息学分析 |
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杜文苹1,2，曹然然1,2，胡晓玉1,2，张晨曦1,2，许春红1,2，杨岚1,2，雷艳茹1,2，康相涛1,2，李文婷1,2*
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| (1.河南农业大学 动物科技学院,郑州 450046;2.河南省农业科学院 神农种业实验室,郑州 450046) |
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| 摘要: |
| 为探究Runt相关转录因子3 （ Runt-related transcription factors 3，RUNX3 ） 基因潜在功能，本研究利用实时荧光定量技术（Quantitative real-time PCR， qRT-PCR）检测鸡RUNX3基因在心脏、肝脏、脾脏、腿肌和十二指肠组织中的表达，通过生物信息学软件构建RUNX3基因的系统进化树，并对该基因所编码的蛋白质理化性质和结构功能进行分析。结果表明：1）鸡RUNX3基因在脾脏和十二指肠表达量较高，显著高于其他组织（P<0.05）。2）核苷酸序列的系统进化分析表明，鸡RUNX3与火鸡的亲缘关系最近，保守性较高。3）理化性质分析显示，鸡RUNX3基因编码蛋白属于稳定、亲水性蛋白；结构域预测发现，该蛋白不存在跨膜结构，含有2个保守结构域，二级结构和三级结构主要由无规卷曲组成，预测含有58个磷酸化位点，31个丝氨酸位点、23个苏氨酸位点和4个酪氨酸位点。4）蛋白互作预测发现，RUNX3与SMAD3、CBFB、GATA3、EP300、SMAD2、CTNNB1等蛋白之间有较强互作关系。综上，鸡RUNX3在脾脏和十二指肠中高表达，其核苷酸序列在禽类中具有高度保守性，RUNX3蛋白属于稳定、亲水性的不跨膜蛋白，RUNX3与SMAD3、CBFB、GATA3等蛋白之间有较强互作关系，本研究为进一步探究鸡RUNX3在分子水平的作用机制提供理论依据。 |
| 关键词: 鸡 RUNX3基因 组织表达谱 生物信息学分析 |
| DOI：10.11841/j.issn.1007-4333.2024.03.11 |
| 投稿时间：2023-08-09 |
| 基金项目:河南省优秀青年科学基金项目（232300421034）；神农种业实验室一流课题（SN01-2022-05） |
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| Tissue expression and bioinformatics analysis of the chicken RUNX3 gene |
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DU Wenping1,2, CAO Ranran1,2, HU Xiaoyu1,2, ZHANG Chenxi1,2, XU Chunhong1,2, YANG Lan1,2, LEI Yanru1,2, KANG Xiangtao1,2, LI Wenting1,2*
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| (1.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China;2.Shennong Laboratory, Henan Academy of Agricultural Sciences, Zhengzhou 450046, China) |
| Abstract: |
| To investigate the potential functions of the Runt-related transcription factor 3 （RUNX3） gene， this study utilized quantitative real-time PCR （qRT-PCR） to detect the expression of the chicken RUNX3 gene in the heart， liver， spleen， leg and duodenum tissues. The evolutionary tree of the RUNX3 gene was constructed using bioinformatics software， and the physicochemical properties and structural functions of the encoded protein were analyzed. The results showed that： 1） The expression level of the chicken RUNX3 gene was significantly higher in the spleen and duodenum compared to other tissues （P<0.05）. 2） Phylogenetic analysis of the nucleotide sequences showed that chicken RUNX3 was most closely related to turkey and highly conserved. 3） Physicochemical property analysis showed that chicken RUNX3 gene-encoded protein was a stable and hydrophilic protein. Structural domain prediction revealed that the protein did not have a transmembrane structure， contained two conserved structural domains， and the secondary and tertiary structure mainly consisted of random curls， and was predicted to contain 58 phosphorylation sites， 31 serine sites， 23 threonine sites， and 4 tyrosine sites. 4） Protein interactions prediction revealed that RUNX3 was associated with SMAD3， CBFB， GATA3， EP300， SMAD2， CTNNB1 and other proteins. In conclusion， chicken RUNX3 was highly expressed in spleen and duodenum， its nucleotide sequence was highly conserved in avian species， RUNX3 protein was a stable， hydrophilic， non-transmembrane protein， and there was a strong interaction between RUNX3 and proteins such as SMAD3， CBFB， and GATA3， etc. The present study provides a theoretical basis for the further study of the mechanism of action of chicken RUNX3 at molecular level. |
| Key words: chicken RUNX3 gene tissue expression profile bioinformatics analysis |
