| 摘要: |
| 为探究远红光强度对黄瓜幼苗形态、光合性状及碳水化合物的影响，明确不同红光（R）/远红光（FR）比值对黄瓜光合相关指标及参数的影响，以‘新津优1号’黄瓜为材料，在固定红蓝光强度及光合有效辐射的基础上，添加3种不同强度的远红光，使得各处理红光∶远红光的比值分别为10（L-FR）、1.2（M-FR）和0.8（L-FR），比较不同处理对于黄瓜幼苗生长及光合的影响。结果表明：增加远红光强度对黄瓜幼苗形态指标影响显著，M-FR和H-FR处理的鲜重较L-FR分别显著增加60.04%和56.60%；H-FR和M-FR处理株高较L-FR分别增加246.43%和200.63%。随着远红光强度增加，3个处理组气孔密度也随之显著增加，且气孔发育关键基因（MUTE和EPF1）相对表达量差异显著，蒸腾速率（E）、胞间CO2浓度（Ci）和气孔导度（Gsw）随之增加；叶片上表皮、栅栏组织、海绵组织及叶片厚度均呈现下降趋势；在一定程度内添加远红光将提高Rubisco酶活化水平。各处理黄瓜幼苗的总氮含量和碳氮比均随着远红光强度的增加而显著下降，L-FR处理黄瓜碳水化合物含量低于H-FR处理。综上，M-FR处理的远红光强度适宜作为远红光补充强度用于工厂化育苗生产中。 |
| 关键词: 远红光 黄瓜幼苗 形态 光合 碳水化合物 |
| DOI：10.11841/j.issn.1007-4333.2024.02.07 |
| 投稿时间：2023-05-07 |
| 基金项目:陕西省技术创新引导专项（2021QFY08-02）；2021年陕西省千亿级设施农业专项（K3030821094）；西藏高原设施蔬菜关键技术创新与集成项目（XZ202202YD0002C）；设施蔬菜瓜果名优品种引进与标准化示范基地建设项目（QYXTZX-AL2023-07） |
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| Effects of adding different intensities of far-red light on the growth and photosynthesis of cucumber seedlings |
|
LI Xue1, ZHAO Shiwen1, ZHANG Guanzhi1, BAO Xingxing1, WU Yongjun2, YANG Zhenchao1*
|
| (1.College of Horticulture, Northwest A & F University, Yangling 712100, China;2.College of Life Science, Northwest A & F University, Yangling 712100, China) |
| Abstract: |
| In order to investigate the effects of far-red light intensity on the morphology， photosynthetic traits and carbohydrates of cucumber seedlings， and to clarify the effects of different red light （R）/far-red light （FR） ratios on the photosynthesis-related indexes and parameters of cucumber， cucumbervariety 'Xinjin You No.1' was taken as the study material. On the basis of fixing the red-blues light intensity and the photosynthetically active radiation， three different kinds of fixed red and blue light intensity and photosynthetically active radiation， three different kinds of far-red light were added to make the ratio of red light：far-red light of each treatment 10 （L-FR）， 1.2 （M-FR） and 0.8 （L-FR）， respectively， aiming to compare the effects of the different treatments on the growth and photosynthesis of cucumber seedlings. The results showed that： Increasing far-red light intensity had a significant effect on the morphological indexes of cucumber seedlings， and the fresh weight of M-FR， and H-FR groups increased significantly by 60.04% and 56.60%， respectively， compared with that of L-FR group； The plant height of H-FR and M-FR groups increased by 246.43% and 200.63%， respectively， compared with that of L-FR. With the increase of far-red light intensity， the stomatal density of the three treatment groups also increased significantly， and the relative expression of key genes of stomatal development （MUTE， EPF1） differed significantly， while their transpiration rate （E）， inter-cellular CO2 concentration （Ci） and stomatal conductance （Gsw） increased； The epidermis of the leaf blade， the fenestrated tissues， spongy tissues， and the thickness of the leaf blade showed a decreasing trend.The total nitrogen content and carbon to nitrogen ratio of cucumber seedlings in all treatments showed a significant decreasing trend with the increase of far-red light intensity； the carbohydrate content of cucumber under L-FR treatment was lower than that of H-FR treatment. In conclusion， the far-red light intensity under M-FR treatment is suitable to be used as a supplemental intensity of far-red light in factory nursery production. |
| Key words: far-red light cucumber seedlings morphology photosynthesis carbohydrate |
