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| 薄壳山核桃-小麦套作系统土壤微生物群落研究 |
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张嘉恬1,2,常君2*,杨水平1*,姚小华2,王开良2,葛翔3
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| (1.西南大学 资源环境学院, 重庆 400716;2.中国林业科学研究院 亚热带林业研究所, 杭州 311400;3.阜阳市新丰种业有限公司, 安徽 阜阳 236000) |
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| 摘要: |
| 为研究薄壳山核桃-小麦套作系统产量效益及土壤微生物群落情况，以薄壳山核桃单作(P)和薄壳山核桃-小麦套作(PW)为研究对象，分析比较2种模式下的产量效益，利用Illumina Miseq高通量测序技术对土壤细菌及真菌多样性、群落结构、标志物种和共现网络进行研究，并探究土壤化学性质与微生物的关系。结果表明:1)套作后坚果产量显著低于单作，总产值显著高于单作；2)套作导致土壤细菌多样性和真菌丰富度显著降低；3)细菌和真菌群落结构因套作显著改变，放线菌门(Actinobacteria)与担子菌门(Basidiomycota)丰度显著提高，酸杆菌门(Acidobacteria)丰度显著降低，微球菌科(Micrococcaceae)、黄色杆菌科(Xanthobacteraceae)、小单孢菌科(Micromonosporaceae)、鞘脂单胞菌属(Sphingomonas)、热酸菌属(Acidothermus)、芽单胞菌属(Gemmatimonas)、假节杆菌属(Pseudarthrobacter)和外瓶霉属(Exophiala)等标志物种于套作土壤中富集；4)套作使细菌网络复杂性增强，真菌网络复杂性减弱；5)属水平细菌及真菌的丰度与土壤化学性质显著相关，pH、有机质、硝态氮、速效磷和全磷驱动细菌群落结构变化，有机质、硝态氮、速效磷和速效钾驱动真菌群落结构变化。本研究结果有助于揭示薄壳山核桃-小麦套作系统的地下生态机制。 |
| 关键词: 土壤微生物 薄壳山核桃 农林复合经营 粮油安全 |
| DOI：10.11841/j.issn.1007-4333.2023.12.08 |
| 投稿时间：2023-03-12 |
| 基金项目:浙江省重点研发计划(2021C02038)；中央级公益性科研院所基本科研业务费专项资金(CAFYBB2020SY014,CAFYBB2017ZA004-8) |
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| Study on soil microbial community of pecan-wheat intercropping system |
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ZHANG Jiatian1,2,CHANG Jun2*,YANG Shuiping1*,YAO Xiaohua2,WANG Kailiang2,GE Xiang3
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| (1.College of Resources and Environment, Southwest University, Chongqing 400716, China;2.Research Institute of Subtropical Forestry, China Academy of Forestry, Hangzhou 311400, China;3.Fuyang Xinfeng Seed Industry Co., Ltd., Fuyang 236000, China) |
| Abstract: |
| In order to study the yield benefit and soil microbial community of pecan-wheat intercropping system, pecan monoculture(P)and pecan-wheat intercropping(PW)were used as the research objects to compare and analyze the yield benefits under the two modes. Illumina Miseq high-throughput sequencing technology was used to study the diversity, community structure, marker species and co-occurrence network of soil bacteria and fungi, and to explore the relationship between soil chemical properties and microorganisms. The results showed that: 1)The yield of pecan nuts after intercropping was significantly lower than that of monoculture, and its total output value was significantly higher than that of monoculture. 2)Intercropping significantly reduced soil bacterial diversity and fungal richness. 3)Both bacterial and fungal microbial community structure were significantly changed by intercropping. The abundance of Actinobacteria and Basidiomycota increased significantly, and the abundance of Acidobacteria decreased significantly. Micrococcaceae, Xanthobacteraceae, Micromonosporaceae, Sphingomonas, Acidothermus, Gemmatimonas, Pseudarthrobacter, Exophiala and other marker species were enriched in the intercropping soil. 4)The complexity of soil bacterial network in intercropping was higher than that in monoculture, and the complexity of fungal network was lower than that in monoculture. 5)The abundance of bacteria and fungi at the genus level was significantly correlated with soil chemical properties. The changes of bacterial community structure were driven by pH, organic matter, nitrate nitrogen, available phosphorus and total phosphorus, and the changes of fungal community structure were driven by organic matter, nitrate nitrogen, available phosphorus and available potassium. The results of this study are helpful to reveal the underground ecological mechanism of pecan-wheat intercropping system. |
| Key words: soil microorganisms pecan agroforestry grain and oil safety |
