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| 高粱自交系萌发期对干旱胁迫的响应及抗旱性评价 |
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张瑞栋1,常嘉荣2,陈海云2,梁晓红1,刘静1,南怀林1,王颂宇1,曹雄1*
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| (1.山西农业大学 经济作物研究所, 太原 030031;2.山西农业大学 农学院, 太原 030031) |
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| 摘要: |
| 为筛选出萌发期抗旱性较强的高粱种质资源，用PEG-6000溶液模拟干旱胁迫，以0.013、0.020、0.025、0.029 mol/L(80、120、150和175 g/L)PEG-6000处理6个高粱自交系，通过对发芽指标的分析，确定高粱萌发期抗旱筛选鉴定的最适浓度，测定118份高粱自交系在此胁迫浓度下的相对发芽率、相对芽长、相对根长、相对芽重、相对根重等指标的变化，通过相关分析、主成分分析、聚类分析，鉴定不同高粱自交系的抗旱性。结果表明:0.025 mol/L(150 g/L)为6个高粱自交系萌发期抗旱性鉴定的最适浓度。对118份高粱自交系萌发期抗旱鉴定发现干旱胁迫对芽鲜重影响最大，对发芽率影响最小；相对芽长与相对根长、相对芽重、相对根重均呈极显著正相关，相关系数(r)分别为0.35、0.78、0.25；相对根长与相对芽重呈极显著正相关，r为0.25，与相对根重呈显著正相关，r为0.23；相对芽重和相对根重呈极显著正相关，r为0.33。各萌发指标的相对值之间存在一定的相关性，抗旱评价信息存在相互交叉。通过主成分分析，明确了不同指标的权重，对萌发指标相对值进行加权运算得到抗旱综合得分。对不同自交系的抗旱综合得分进行聚类分析，将118份高粱自交系萌发期的抗旱性分为五大类:‘20LCS209’等8个自交系为极端抗旱型自交系，‘4126’等31个自交系为抗旱自交系，‘10036’等41个自交系为抗旱中间型自交系，‘20LCS167’等26个自交系为干旱敏感自交系，‘20LCS203’等12个自交系为干旱极端敏感自交系。 |
| 关键词: 高粱 萌发期 抗旱 主成分分析 聚类分析 |
| DOI：10.11841/j.issn.1007-4333.2023.11.05 |
| 投稿时间：2023-02-25 |
| 基金项目:山西省高等学校科技创新项目(2021L149)；国家现代农业产业技术体系(CARS-06-14.5-B10)；国家自然科学基金(32101846) |
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| Response to drought stress and drought resistance evaluation of sorghum inbred lines at germination stage |
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ZHANG Ruidong1,CHANG Jiarong2,CHEN Haiyun2,LIANG Xiaohong1,LIU Jing1,NAN Huailin1,WANG Songyu1,CAO Xiong1*
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| (1.Institute of Industrial Crops, Shanxi Agricultural University, Taiyuan 030031, China;2.College of Agriculture, Shanxi Agricultural University, Taiyuan 030031, China) |
| Abstract: |
| To screen drought-resistant germplasm for sorghum during the germination period, PEG-6000 was used to simulate drought stress. Six sorghum inbred lines were taken as study material and treated with 0. 013, 0. 020, 0. 025 and 0. 029 mol/L(80, 120, 150 and 175 g/L)PEG-6000 aqueous solutions in germination trials, respectively. A total of 118 sorghum lines were studied for germination under stress in 0. 025 mol/L(150 g/L)PEG-6000 aqueous solution and analyzed for changes in relative germination rate, relative shoot length, relative root length, relative shoot weight and relative root weight indices. The results showed that: The PEG-6000 solution of 0. 025 mol/L(150 g/L)was identified as the drought resistant identification concentration for six sorghum inbred lines during germination. All of the 118 sorghum inbred lines were identified for drought resistance. It was found that drought stress had the greatest effect on shoot fresh weight and the least effect on germination rate. The results of correlation analysis showed that the relative shoot length was highly significantly positively correlated with relative root length, relative shoot weight and relative root weight, with correlation coefficients(r)of 0. 35, 0. 78 and 0. 25, respectively; relative root length was highly significantly positively correlated with relative shoot weight, with r of 0. 25, relative root length was significantly positively correlated with relative root weight, with r of 0. 23; The relative shoot weight and relative root weight were highly significantly positively correlated, with r of 0. 33. There was certain correlation between the relative values of the germination indicators and the information on drought tolerance evaluation crossed each other. Principal component analysis was carried out on the relative values of germination indicators to clarify the weights of different indicators. The relative values of germination indicators were weighted to obtain a composite score for drought tolerance. The drought tolerance scores of the different inbred lines were clustered and the 118 sorghum inbred lines were finally classified into 5 major categories as follows: Eight inbred lines such as ‘20LCS209' as extremely drought tolerant, 31 inbred lines such as ‘4126' as drought tolerant, 41 inbred lines such as ‘10036' as drought intermediate, 26 inbred lines such as ‘20LCS167' as drought sensitive and 12 inbred lines such as ‘20LCS203' as extremely drought sensitive. |
| Key words: sorghum germination period drought resistance principal component analysis cluster analysis |
