| 摘要: |
| 为探究马铃薯转录因子StNAC043基因的功能，克隆马铃薯转录因子StNAC043基因，利用农杆菌介导法将其转入马铃薯‘东农310’基因组，使其过量表达。转基因植株经无性系扩繁后，测定转基因株系的根和茎的生长指标、叶绿素相对含量(SPAD)以及荧光参数(Fv/Fm、ETRmax)，并以转录组测序结合qRT-PCR验证分析其下游调控基因。结果表明:StNAC043基因过量表达可以显著促进转基因马铃薯根系和茎的生长、显著提高转基因植株叶片SPAD、Fv/Fm、ETRmax；并且转录因子StNAC043可以调控捕光色素结合蛋白基因StCAB1和StCAB2的表达。综上，马铃薯转录因子StNAC043基因对马铃薯幼苗的生长和光合生理调控具有重要作用。 |
| 关键词: 马铃薯 转录因子 StNAC043 根系 荧光参数 |
| DOI：10.11841/j.issn.1007-4333.2023.10.06 |
| 投稿时间：2023-01-03 |
| 基金项目:现代农业产业技术体系建设专项(CARS-09)；寒地粮食作物种质创新与生理生态教育部重点实验室开放课题(CXSTOP202205) |
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| Cloning and physiological function analysis of potato transcription factor StNAC043 gene |
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TANG Xinhua1,2,QU Zicheng1,LI Shiwei1,LIU Yulin1,SHI Ying1*
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| (1.Agricultural College, Northeast Agricultural University, Harbin 150030, China;2.National Research Center of Soybean Engineering and Technology, Harbin 150028, China) |
| Abstract: |
| To reveal the function of potato transcription factor StNAC043 gene, a potato transcription factor StNAC043 gene was cloned and transferred into potato ‘Dongnong 310' genome by agrobacterium-mediated method to overexpress the gene. After clonal propagation of transgenic plants, the root and stem growth indexes, chlorophyll relative content(SPAD)and fluorescence parameters(Fv/Fm and ETRmax)of transgenic lines were measured, and the downstream regulatory genes were verified by transcriptome sequencing combined with qRT-PCR. The results showed that the overexpression of StNAC043 gene significantly promoted the growth of roots and stems of transgenic potato, significantly increased the SPAD, Fv/Fm and ETRmax of transgenic plants, and the transcription factor StNAC043 regulated the expression of light-harvesting pigment binding protein genes StCAB1 and StCAB2. In conclusion, the potato transcription factor StNAC043 gene plays an important role in potato seedling growth and photosynthetic physiological regulation. |
| Key words: potato transcription factor StNAC043 root fluorescence parameters |
