| 摘要: |
| 为探究外源茉莉酸(Jasmonic acid，JA)对不同干旱胁迫下燕麦转录组的影响，本研究测定轻度(0.017 mol/L PEG-6000处理)和重度干旱胁迫(0.034 mol/L PEG-6000处理)处理及再添加JA处理的‘蒙燕1号’转录组，并对表达显著差异基因(DEGs)进行GO和KEGG功能富集分析。结果表明:GO功能富集分析显示，不同干旱胁迫处理下，外源JA诱导的DEGs均主要富集在膜、催化酶、转移酶和对刺激反应等代谢途径。KEGG功能富集分析显示，在未进行干旱胁迫条件下(CK)，外源JA诱导1 955个基因表达上调，4 666个下调；DEGs主要富集的通路为代谢途径和次生代谢物的合成，其中苯丙素的生物合成通路基因表达发生显著变化。轻度干旱胁迫下，外源施用JA诱导1 574个基因表达上调，933个基因下调；DEGs主要富集的通路为次生代谢的生物合成、植物激素信号转导、精氨酸和脯氨酸代谢通路，其中脱落酸和水杨酸信号转导、精氨酸和脯氨酸代谢通路中基因表达均上调；重度干旱胁迫下，外源JA诱导223个基因表达上调，456个下调；DEGs显著富集的只有鞘脂代谢通路。与未施用JA相比，不同干旱胁迫下，施用JA均可诱导细胞分裂素(CTK)代谢通路中CTK的N-糖基化基因(UGT73C5)上调和氧化酶/脱氢酶基因(CKX11)下调，以及脱落酸(ABA)信号转导相关基因(THI1.3)和未知功能基因(AT1G71250)表达发生显著变化。综上，轻度和重度干旱胁迫下，外源JA能诱导燕麦响应干旱胁迫的CTK和ABA相关基因表达发生显著变化。 |
| 关键词: 燕麦 外源JA 干旱胁迫 转录组测序 |
| DOI：10.11841/j.issn.1007-4333.2023.08.08 |
| 投稿时间：2022-10-21 |
| 基金项目:国家燕麦荞麦产业技术体系(CARS-07-A-4)；内蒙古自治区科技重大专项(2021SZD0017)；内蒙古农牧业创新基金(2022CXJJN04) |
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| Effects of exogenous jasmonic acid on the transcriptome of oat under different water conditions |
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ZHANG Zhifen1,FU Xiaofeng1,CUI Siyu1,FU Ying2,LIU Junqing1,YANG Haishun1
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| (1.Institute of Specialty Crops, Academy of Agriculture and Animal Husbandry of Inner Mongolia Autonomous Region, Hohhot 010031, China;2.Laboratory of crop breeding and cultivation, Hinggan League Agricultural and Animal Husbandry Sciences Research Institute, Ulanhot 137400, China) |
| Abstract: |
| In order to explore the effect of exogenous jasmonic acid(JA)on the transcriptome of oat under different drought stress, the transcriptome of ‘Mengyan 1' treated with JA after mild(0. 017 mol/L PEG-6000 treatment)and severe drought stress(0. 034 mol/L PEG-6000 treatment)was determined. GO and KEGG functional enrichment analysis were adopted to investigated the significant differentially expressed genes(DEGs). GO function enrichment analysis showed that JA mainly affected membrane, catalytic enzyme, transferase and response to stimulation under different water conditions. The results of KEGG functional enrichment analysis showed that 1 955 up-regulated genes and 4 666 down-regulated genes were induced by JA under CK. DEGs was significantly enriched in metabolic pathway and secondary metabolite synthesis pathway. Gene expression pattern in phenylpropanoid biosynthesis pathway changed significantly. A total of 1 574 genes up-regulated and 933 genes down-regulated were induced by JA under mild drought stress. DEGs was significantly enriched in the biosynthesis of secondary metabolism, plant hormone signal transduction, arginine and proline metabolic pathways. Genes involved in ABA and JA signal transduction and arginine and proline metabolism were up-regulated. About 223 genes up-regulated and 456 down-regulated were induced by JA under severe drought stress. Sphingolipid metabolic pathway was significantly enriched. Compared with the untreated JA, the application of JA under different drought stress induced the up-regulation of CTK N-glycosylation gene(UGT73C5)and the down-regulation of oxidase/dehydrogenase gene(CKX11)in the cytokinin(CTK)metabolic pathway, as well as the significant changes in the expression of abscisic acid(ABA)signal transduction related gene(THI1. 3)and unknown functional gene(AT1G71250). To sum up, under mild and severe drought stress, exogenous JA can induce significant changes in the expression of oat CTK and ABA-related genes responsive to drought stress. |
| Key words: Avena sativa L. exogenous jasmonic acid drought stress transcriptome |
