| 摘要: |
| 为了解载体介导噬菌体入胞及抗胞内寄生菌的最新研究进展，利用文献综述法检索近年载体介导噬菌体入胞的相关文献，从功能发挥、试验技术及应用前景等方面对可应用于介导噬菌体入胞的载体进行整理讨论。结果表明:1)噬菌体作为一类能够感染和裂解细菌的病毒，其天然存在的抗菌特性使其作为抗生素替代物的研究广泛存在，但其靶向胞内寄生菌的基础研究主要集中在国外，国内鲜有报道。2)与游离噬菌体相比，以无机纳米微粒、脂质体等作为载体介导噬菌体入胞，能够防止噬菌体被宿主免疫系统清除和免受细胞内环境影响。3)脂质体因其作为载体可有效提高噬菌体入胞效率及杀菌效率，现已成为研究最广泛的噬菌体入胞载体之一。因此，本文综述近年来国内外关于载体介导噬菌体入胞及抗胞内寄生菌研究进展，详细阐述不同介导噬菌体入胞的载体特性、入胞机制以及如何提高入胞效率等方面的最新研究，并针对目前存在的问题和发展前景进行展望，以期为更有效地利用载体投递系统高效投递噬菌体解决细胞内细菌感染的实际问题提供参考与理论支撑。 |
| 关键词: 噬菌体 载体介导 胞内寄生菌 抗菌活性 |
| DOI：10.11841/j.issn.1007-4333.2023.06.02 |
| 投稿时间：2022-07-31 |
| 基金项目:国家自然科学基金青年科学基金项目(31802226)；黑龙江省自然科学基金联合引导项目(LH2022C072)；黑龙江省牛病防制重点实验室开放课题(PCBD201710) |
|
| Recent advances in bacteriophage entry into cells by carrier delivery and against intracellular bacteria |
|
SONG Jun,YUAN Qingxin,YANG Shubo,ZHOU Zhixin,SUN Dongbo*
|
| (College of Animal Science and Veterinary Medicine/Key Laboratory of Bovine Disease Control in Northeast China ofMinistry of Agriculture and Rural Affairs/Key Laboratory of Prevention and Control of Bovine Diseases ofHeilongjiang Province, Heilongjiang Bayi Agricultural University, Daqing 163319, China) |
| Abstract: |
| In order to improve the antibacterial and therapeutic effect of bacteriophages against intracellular bacteria, the literature review method was used to retrieve the relevant literatures on carrier mediated phages entry into cells in recent years. The functional development, experimental techniques and application prospects of carrier mediated into cells were summarized and discussed. The results showed that: 1)Bacteriophages, as a class of viruses, can infect and lyse bacteria. The natural antibacterial properties of phages make them widely studied as alternatives to antibiotics. However, the basic research of bacteriophage on intracellular bacteria is abundant in foreign countries, but few in China. 2)Compared with free phages, using inorganic nanoparticles, liposomes as carriers to mediate phage delivery can effectively improve the efficiency of phage entry into cells, and prevent phages from being cleared by the host immune system and avoid the influence of intracellular environment. 3)Liposome has become the most widely studied phage carrier into cell. Because it can effectively improve phage entry efficiency and bactericidal efficiency as a carrier. Therefore, this paper reviews the recent research progress of carrier mediated phage entry and anti-intracellular bacteria, and expounds the different carrier mediated characteristics, the mechanism of entry into the cell and how to improve the efficiency of entry. Finally, the present problems and development prospects are prospected, in order to provide reference and theoretical support for solving practical problems of intracellular bacterial infection by efficient delivery of phages with carrier delivery system. |
| Key words: bacteriophage carrier delivery system intracellular bacteria antibacterial activity |
