| 本文已被：浏览 563次 下载 4329次 |
码上扫一扫！ |
| 铜(II)-多酚纳米复合物对水培营养液中藻类生长的抑制 |
|
王明耀1,杨晓2,黄涛2,王晓玲1,3,4*,杨其长2,郭俊凌1,3,4*
|
|
|
| (1.四川大学 生物质与皮革工程系, 成都 610065;2.中国农业科学院 都市农业研究所/成都国家农业科技中心, 成都 610213;3.四川大学 制革清洁技术国家工程实验室, 成都 610065;4.四川大学 皮革化学与工程教育部重点实验室, 成都 610065) |
|
| 摘要: |
| 为研究铜(II)-多酚纳米复合物对水培营养液中藻类生长的抑制作用及对生菜的影响，将金属离子(Cu2+)和天然植物多酚(杨梅单宁BT、橡椀单宁VT、塔拉单宁Tr、单宁酸TA)自组装形成铜(II)-多酚纳米复合物，设置铜(II)-多酚纳米复合物抑制小球藻试验和实际水培生菜试验。结果表明:1)多酚诱导的界面相互作用促使铜(II)-多酚纳米复合物能够黏附于藻类细胞表面并缓慢释放铜离子，从而引起了藻细胞的氧化损伤和藻细胞的死亡，可实现长期有效的抑藻效果。其中，铜与杨梅单宁BT形成的CuII-BT使用7 d后，对小球藻的抑制率仍然可达到99%。2)Cu2+与缩合类植物多酚(杨梅单宁BT、橡椀单宁VT)自组装形成的CuII-BT和CuII-VT对藻类生长的抑制能力优于Cu2+与水解类植物多酚(塔拉单宁Tr、单宁酸TA)自组装形成的CuII-Tr和CuII-TA。3)铜(II)-多酚纳米复合物能够在实际生产中抑制营养液中藻类的生长，并且与对照相比，水培生菜鲜重没有差异，证明了铜(II)-多酚纳米颗粒具有良好的生物安全性。因此，在实际水培生产中，自组装的铜(II)-多酚纳米颗粒具有抑制藻类生长的能力，可缓解由藻类过度繁殖造成的粮食作物减产问题。 |
| 关键词: 植物多酚 抑藻 铜离子 定向缓释作用 水培技术 |
| DOI：10.11841/j.issn.1007-4333.2023.05.10 |
| 投稿时间：2022-08-03 |
| 基金项目:国家海外高层次人才计划青年项目；国家自然科学基金项目(22178233,22108181)；国家重点研发计划(2022YFA0912800)；高分子材料工程国家重点实验室(SKLPME2020-03-01)；四川省重点研发项目(2022YFN0070)；农业科技创新计划(ASTIP-CAAS,34-IUA) |
|
| Inhibition effects of copper(II)-phenolic nanocomplexes on the growth of algae in hydroponic nutrient solution |
|
WANG Mingyao1,YANG Xiao2,HUANG Tao2,WANG Xiaoling1,3,4*,YANG Qichang2,GUO Junling1,3,4*
|
| (1.College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China;2.Institute of Urban Agriculture/Chengdu National Agricultural Science and Technology Center, Chinese Academy of Agricultural Sciences, Chengdu 610065, China;3.National Engineering Laboratory for Clean Technology in Leather Industry, Sichuan University, Chengdu 610065, China;4.Key Laboratory of Leather Chemistry and Engineer of Ministry of Education, Sichuan University, Chengdu 610065, China) |
| Abstract: |
| The inhibition effects of copper(II)-phenolic nanocomplexes on algal growth and its effect on lettuce in hydroponics were studied. In this study, natural polyphenols(Bayberry tannin, Valonia tannin, Tara tannin, and Tannin acid)and functional metal ions(copper ions)were used as building blocks to engineer self-assembled copper(II)-phenolic nanocomplexes, and the inhibition experiment of copper(II)-phenolic nanocomplexes for Chlorella vulgaris and application of copper(II)-phenolic nanocomplexes on actual hydroponic lettuce were designed. The results showed that: 1)The polyphenol-induced interface interaction enabled the attachment of copper(II)-phenolic nanocomplexes on the surface of algal cells, which continuously and precisely delivered metal ions(copper ions)to algae, resulting in oxidative damage and cell death. Thus, the copper(II)-phenolic nanocomplexes presented long-lasting inhibition for algae, and the corresponding inhibition rate of CuII-BT still reached 99% on the 7th day for C. vulgaris. 2)The algal inhibition experiments showed that the inhibition ability of CuII-BT and CuII-VT for C. vulgaris was better than that of CuII-Tr and CuII-TA. 3)Plant experiments showed that copper(II)-phenolic nanocomplexes could inhibit algae during actual production, and there was no difference in fresh weight of lettuce seedlings compared with the control group. These results demonstrate the copper(II)-phenolic nanocomplexes had greater biosafety. Thus, the self-assembled copper(II)-phenolic nanocomplexes could inhibit algal blooms in actual hydroponic production, which contributed to reduce the problem of loss of crop yield caused by algal blooms. |
| Key words: plant polyphenol algal growth inhibition, copper ions target delivery system hydroponic technology |
