欢迎访问本编辑部网站！

本文已被：浏览 437次下载 1350次	码上扫一扫！
绵羊繁殖相关编码RNA和非编码RNA的研究进展
刘在霞^1,2,张景萍³,付绍印⁴,何小龙⁴,王标⁴,何江峰⁴,白音巴图^1,2,韩程志^1,2,马凤英^1,2,张文广^1,2,5,石彩霞^1,刘永斌^6
0 字体:加大+\|默认\|缩小-
(1.内蒙古农业大学动物科学学院, 呼和浩特 010018;2.内蒙古自治区农业基因组大数据工程研究中心, 呼和浩特 010018;3.赤峰市农牧业综合行政执法支队元宝山大队, 内蒙古赤峰 024000;4.内蒙古自治区农牧业科学院畜牧研究所, 呼和浩特 010031;5.内蒙古农业大学生命科学学院, 呼和浩特 010011;6.内蒙古大学生命科学学院, 呼和浩特 010021)

摘要:

随着分子生物学和生物信息学的发展及应用，已有诸多研究从影响绵羊卵巢发育、排卵、产羔、睾丸发育和精子发生等主要繁殖活动相关编码RNA和非编码RNA方面着手，对绵羊繁殖相关的编码和非编码RNA进行筛选和鉴定。本文对影响绵羊繁殖的编码RNA和非编码RNA研究成果进行总结梳理，共整理了61个关键mRNAs、9个miRNAs、17个lncRNAs和4个circRNAs，并进一步阐述RNA的发展历史和生物学功能，以及编码RNA与非编码RNA构成调控绵羊繁殖的复杂ceRNA网络。结果表明:1)不同年龄公羊睾丸和母羊卵巢在繁殖过程中mRNA呈动态变化；2)非编码RNA与DNA、RNA或蛋白质之间相互作用，调控转录、翻译以及翻译后修饰等过程，实现不同品种、年龄和饲喂水平绵羊对精子发生和卵泡发育等过程的调控；3)编码RNA和非编码RNA形成稳定的ceRNA调控机制使繁殖活动趋于动态平衡。本综述为挖掘绵羊繁殖性状分子标记基因、开展分子育种工作、改善绵羊繁殖性能及提高羊产业经济效益打下基础。

关键词: 绵羊繁殖性状编码RNA 非编码RNA

DOI：10.11841/j.issn.1007-4333.2023.04.11

投稿时间：2022-07-27

基金项目:国家重点研发计划(2021YFD1200905)；内蒙古自治区科技重大专项(2020ZD0003)；内蒙古自治区科技重大专项(2019ZD016)；内蒙古自治区科技计划(2021GG0012)；财政部和农业农村部:国家现代农业产业技术体系资助(CARS-38)

Research progress on the coding RNA and non-coding RNA related to sheep reproduction

LIU Zaixia^1,2,ZHANG Jingping³,FU Shaoyin⁴,HE Xiaolong⁴,WANG Biao⁴,He Jiangfeng⁴,Baiyinbatu^1,2,HAN Chengzhi^1,2,MA Fengying^1,2,ZHANG Wenguang^1,2,5,SHI Caixia^1*,LIU Yongbin^6*

(1.College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China;2.Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Hohhot 010018, China;3.Chifeng City Agriculture and Animal Husbandry Comprehensive Administrative Law EnforcementDetachment Yuanbaoshan Brigade, Inner Mongolia Chifeng 024000, China;4.Animal Husbandry Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;5.College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010011, China;6.School of Life Science, Inner Mongolia University, Hohhot 010021, China)

Abstract:

With the development and application of molecular biology and bioinformatics, many studies have screened and identified the coding and non-coding RNA related to sheep reproduction from the aspects of coding RNA and non-coding RNA related to the main reproductive activities such as ovarian development, ovulation, lambing, testicular development and spermatogenesis. In this study, the research of coding RNA and non-coding RNA affecting sheep reproduction were summarized, and 61 key mRNAs, 9 miRNAs, 17 lncRNAs and 4 circRNAs were sorted out. The development history and biological functions of RNA were further elaborated, and the coding RNA and non-coding RNA constituted a complex ceRNA network regulating sheep reproduction. The results showed that: 1)The mRNA of ram testis and ewe ovary at different ages showed dynamic changes during reproduction; 2)The interaction between non-coding RNA and DNA, RNA or protein regulates the processes of transcription, translation and post-translational modification, and realizes the regulation of spermatogenesis and follicular development in sheep of different breeds, ages and feeding levels. 3)The ceRNA regulatory mechanism formed by coding RNA and non-coding RNA, stable ceRNA regulatory mechanism makes the reproductive activity tend to a dynamic balance. This review aims to lay a foundation for excavating molecular marker genes of sheep reproductive traits, carrying out molecular breeding work, improving sheep reproductive performance and improving the economic benefits of the sheep industry.

Key words: sheep reproductive traits coding RNA non-coding RNA