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| 胚蛋注射25-羟基胆钙化醇对樱桃谷雏鸭孵化性能、早期发育与血清生化指标的影响 |
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李龙1,卫城朝1,张伟1,吴凤明2,蔡锋隆2,秦顺义1,李留安1,杨华1,张建斌1*
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| (1.天津农学院 动物科学与动物医学学院/天津市农业动物繁育与健康养殖重点实验室, 天津 300384;2.天津市广源畜禽养殖有限公司, 天津 301824) |
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| 摘要: |
| 为研究胚蛋注射25-OH-D3对雏鸭孵化性能、1~7 d生长发育和血清生化指标的影响，本研究选取60周龄樱桃谷种鸭蛋200枚，随机分为5组，每组8个重复，每个重复5枚种蛋。在孵化第13.5天时，A组不做处理，B组注射0.2 mL 0.85%的生理盐水，C、D、E组分别注射相同体积不同浓度的25-OH-D3溶液。出壳后，饲喂基础饲粮，试验期7 d，计算每组的出壳率，测定体重和血清生化指标。结果表明:1)与A组相比，试验C、D、E组雏鸭的孵化率分别显著提高了4.79%、5.54%和3.37%(P<0.05)。试验C、D、E组对7 d雏鸭的体重无显著影响(P>0.05)，试验D组3 d雏鸭的体重显著增加了13.19%，1 d雏鸭的体重显著提高了1.65%(P<0.05)。2)与A组相比，C、D、E组对雏鸭的胫骨发育无显著影响(P>0.05)。3)与A组相比，试验C、D、E组显著提高了雏鸭血清中生长激素和胰岛素样生长因子I的水平(P<0.05)。试验D、E组雏鸭血清中25-羟基胆钙化醇的水平分别显著提高了56.16%和40.16%(P<0.05)。试验C组雏鸭血清中磷的水平提高了22.20%(P<0.05)。4)与A组比，C、D、E组显著降低了雏鸭血清中的LDL水平(P<0.05)。C组显著提高了雏鸭血清中ALB的含量(P<0.05)。D组显著提高了雏鸭血清中ALP的活性和GLU的水平(P<0.05)，E组显著降低了雏鸭血清中ALP和AST的活性(P<0.05)。C、D组显著提高了雏鸭血清中AST的活性(P<0.05)。综上，胚蛋注射25-OH-D3可提高雏鸭的孵化率和免疫力，其最佳添加剂量为0.8 μg/枚，并促进早期发育，为后期发育提供更好的基础。这提示胚蛋注射25-OH-D3有利于促进雏鸭胚胎发育，并对雏鸭机体健康发挥潜在的积极作用。 |
| 关键词: 樱桃谷雏鸭 25-羟基胆钙化醇 胚蛋注射 早期发育 |
| DOI：10.11841/j.issn.1007-4333.2023.03.11 |
| 投稿时间：2022-06-20 |
| 基金项目:天津市科技计划项目(21ZYCGSN00510)；天津市科技重大专项与工程项目(20ZYCGSN00300,18ZXBFNC00310)；天津市自然科学基金重点项目(20JCZDJC00170)；天津市“131”创新型人才团队建设项目(20180318) |
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| Effects of in ovo injecting 25-hydroxycholecalciferol on hatchability, early development and serum biochemical indexes of Cherry Valley ducklings |
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LI Long1,WEI Chengchao1,ZHANG Wei1,WU Fengming2,CAI Fenglong2,QIN Shunyi1,LI Liuan1,YANG Hua1,ZHANG Jianbin1*
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| (1.College of Animal Science and Veterinary Medicine/Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, Tianjin Agricultural University, Tianjin 300384, China;2.Tianjin Guangyuan Livestock and Poultry Breeding Co., Ltd., Tianjin 301824, China) |
| Abstract: |
| To study the effects of embryo egg injection of 25-OH-D3 on hatchability, 1 to 7 days growth and serum biochemical indexes of ducklings, 200 60-week-old cherry valley duck eggs were randomly divided into 5 groups, with 8 replicates for each group and 5 hatching eggs for each replicate. On the 13. 5th day of incubation, no treatment for group A, while group B was injected with 0. 2 mL 0. 85% normal saline, group C, D and E were injected with the same volume of 25-OH-D3 solution with different concentrations. After shelling, the basic diet was fed for 7 days. The hatching rate of each group was calculated, and the body weight and serum biochemical indexes were measured. The results showed that: 1)Compared with group A, the hatchability of ducklings in groups C, D and E increased by 4. 79%, 5. 54% and 3. 37%, respectively(P<0. 05). Group C, D and E had no significant effect on the bodyweight of 1-day-old and 7-day-old ducklings(P>0. 05). The weight of 3-day-old ducklings in group D increased significantly by 13. 19%, the weight of 1-day-old ducklings in group D increased significantly by 1. 65%(P<0. 05). 2)Compared with phase A, groups C, D and E had no significant effect on the development of tibia of ducklings(P>0. 05). 3)Compared with group A, the levels of growth hormone and insulin-like growth factor I in serum of ducklings in groups C, D and E were significantly increased(P<0. 05). The levels of 25-hydroxycholecalciferol in serum of ducklings in groups D and E increased by 56. 16% and 40. 16%, respectively(P<0. 05). The level of serum phosphorus of ducklings in group C increased by 22. 20%(P<0. 05). 4)Compared with group A, groups C, D and E significantly reduced the levels of LDL in serum of duckling(P<0. 05). Group C significantly increased the content of ALB in the serum of duckling(P<0. 05). Group D significantly increased the activity of ALP and GLU in serum of duckling(P<0. 05)while group E significantly decreased the activity of ALP and AST in serum of duckling(P<0. 05). Group C and D significantly increased the activity of AST in serum of duckling(P<0. 05). Therefore, the injection of 25-OH-D3 into the embryo egg could improve the hatchability and immunity of ducklings, . The optimal additive amount is 0. 8 μg/piece, and promote early growth and development, and provide a better foundation for later development. These results suggest that the injection of 25-OH-D3 in ovo is beneficial to the development of ducklings, and plays a potential positive role in the health of ducklings. |
| Key words: cherry valley ducklings 25-hydroxylcholecalciferol in ovo injection early development |
