| 摘要: |
| 为了开发牛骨胶原蛋白肽(BBP)的抗疲劳功能，对BBP的氨基酸组成和肽段序列进行表征；选取16只7周龄、SPF级的Balb/c小鼠并随机分为2组，包括对照组(生理盐水)和肽干预组(w(BBP)=200 mg/kg))，每日定时灌胃。BBP干预4周后，测定小鼠的负重游泳时间和运动后血清代谢积累物含量。BBP干预18周后，测定血清氧化因子、炎症因子含量和肠道菌群组成。结果表明:1)BBP疏水性氨基酸比例为61.37%，大部分肽段来源于蛋白P02453(I型胶原蛋白的α1链)。2)BBP干预后小鼠负重游泳时间显著提高，且运动后血氨浓度显著降低、谷胱甘肽浓度显著升高(P<0.05)，表明了BBP潜在的抗疲劳能力。3)BBP干预后，在非运动状态下小鼠血清SOD和GSH-Px显著升高，MDA浓度显著降低(P<0.05)，表明了BBP潜在的抗氧化能力；在免疫方面，BBP干预后小鼠血清抗炎因子IL-10和促炎因子IL-6、TNF-α浓度均显著下降(P<0.05)，主要的免疫器官胸腺指数显著下降(P<0.01)，脾指数显著上升(P<0.05)；在粪便中，潜在的有益菌Akkermansia、Lactococcus在肽干预后上调。综上，BBP具有潜在的抗疲劳作用，这种作用可能是通过调节体内抗氧化能力以及肠道菌群结构实现的。 |
| 关键词: 肽 抗疲劳 抗氧化 炎症 肠道菌群 |
| DOI：10.11841/j.issn.1007-4333.2023.03.07 |
| 投稿时间：2022-06-20 |
| 基金项目:国家重点研发计划(2017YFD0400200) |
|
| Characterization of bovine bone collagen peptide and its anti-fatigue effect on Balb/c mice |
|
FENG Ruifang1,ZOU Xiaoxiao1,WANG Kai1,LIU Huaigao2,HONG Hui1,LUO Yongkang1,TAN Yuqing1*
|
| (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2.Anhui Guotai Biotechnology Co., Ltd., Xuancheng 242100, China) |
| Abstract: |
| To exploit the anti-fatigue function of bovine bone collagen peptide(BBP), the amino acid composition and peptide sequence of BBP were characterized. Sixteen 7-week-old SPF Balb/c mice were divided into two groups randomly including the control group(normal saline)and the peptide intervention group(w(BBP)=200 mg/kg)), and all were given daily oral gavage. After 4 weeks of BBP intervention, the weight-bearing swimming time and post-exercise metabolic accumulation content of mice were determined. After 18 weeks of BBP intervention, the content of serum oxidant and inflammatory factors, and intestinal flora composition were determined. The results showed that: 1)The proportion of hydrophobic amino acids was 61. 37%. Most of the peptides belonged to the protein P02453(α1 chain of type I collagen). 2)After BBP intervention, the weight-bearing swimming time of mice was significantly increased, and the serum ammonia concentration was significantly decreased, the glutathione concentration was significantly increased(P<0. 05), indicating the potential anti-fatigue effect of BBP. 3)After BBP intervention, the serum superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)of mice in a non-exercise state were significantly increased, and malondialdehyde(MDA)was significantly decreased(P<0. 05), indicating the potential antioxidant capacity of BBP; In terms of immunity, the concentration of anti-inflammatory factor IL-10 and pro-inflammatory factor IL-6, TNF-α was significantly decreased(P<0. 05), the main immune organ thymus index decreased significantly(P<0. 01), and the spleen index increased significantly(P<0. 05). In feces, the potential beneficial bacteria Akkermansia and Lactococcus were up-regulated after peptide intervention. Taken together, BBP has a potential anti-fatigue effect, which may be achieved by regulating the antioxidant capacity in vivo and the structure of gut microbiome. |
| Key words: peptides antifatigue antioxidant inflammation gut microbiome |
