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荷斯坦牛血浆催乳素浓度遗传参数估计及其与PRL、PRLR和TRH基因关联分析
高清1,胡丽蓉1,张海亮1,张帆1,杨圳1,林逍然1,徐青2,郭刚3,刘巧香4,王雅春1*
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(1.中国农业大学 动物科技学院/农业农村部动物遗传育种与繁殖(家畜)重点实验室/畜禽育种国家工程实验室, 北京 100193;2.北京交通大学 生命科学与生物工程研究院, 北京 100044;3.北京首农畜牧发展有限公司, 北京 100029;4.北京生物种业创新联合体, 北京 101206)
摘要:
为探究奶牛血浆催乳素(Prolactin,PRL)浓度的群体特征及其在不同环境条件和生理阶段下的变化规律,估计牛血浆PRL浓度的遗传参数,探究PRLPRLRTRH基因多态性与血浆PRL浓度之间的相关性,本研究于2014和2017年对北京地区某规模化牧场的472头泌乳期荷斯坦牛进行血浆PRL浓度测定,共获得1 169条数据。首先,采用固定模型分析采样季节、胎次、挤奶前后和泌乳阶段对奶牛血浆PRL浓度的影响;然后,采用重复力动物模型估计血浆PRL浓度的遗传参数;此外,通过扫描PRLPRLRTRH基因的多态位点,对血浆PRL浓度进行基于多态位点和单倍型的关联分析。结果显示:1)泌乳期荷斯坦牛血浆PRL平均浓度为224.29 mIU/L,胎次、采样季节和泌乳阶段对血浆PRL浓度有显著或极显著影响,血浆PRL浓度随胎次和泌乳阶段的推移而逐渐升高,春季和夏季时奶牛血浆PRL浓度极显著高于秋季和冬季(P<0.01);2)血浆PRL浓度的遗传力为0.02,为低遗传力性状;3)PRLPRLR基因基因中未发现与血浆PRL浓度显著相关的SNP位点,TRH基因的rs137596325、rs109144892、rs108991567、rs109468305和rs109330999与血浆PRL浓度显著或极显著相关;4)对3个基因的SNP进行连锁不平衡分析发现,PRLR基因上的单倍型块与血浆PRL浓度具有显著相关的趋势(P<0.1),TRH基因中的2个单倍型块与血浆催乳素浓度显著相关。综上,本研究获得了荷斯坦牛血浆PRL浓度的群体特征、遗传参数,探究了与血浆PRL浓度分泌相关基因的多态性及与其显著关联的分子标记,为深入理解血浆PRL浓度的遗传基础提供参考。
关键词:  奶牛  血浆催乳素  遗传分析  PRL  PRLR  TRH
DOI:10.11841/j.issn.1007-4333.2023.02.10
投稿时间:2022-04-21
基金项目:财政部和农业农村部:国家现代农业产业技术体系资助(CARS-36);长江学者和创新团队发展计划(IRT_15R62);西藏自治区区域协同创新专项(QYXTZX-LS2021-01)
Genetic parameters of plasma prolactin concentration and its association with gene PRL, PRLR and TRH in Holstein cattle
GAO Qing1,HU Lirong1,ZHANG Hailiang1,ZHANG Fan1,YANG Zhen1,LIN Xiaoran1,XU Qing2,GUO Gang3,LIU Qiaoxiang4,WANG Yachun1*
(1.Key Laboratory of Animal Genetics, Breeding and Reproduction(MARA), National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;2.College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing 100044, China;3.Beijing Sunlon Livestock Development Company Limited, Beijing 100029, China;4.Beijing Innovation Consortium of Bio-breeding, Beijing 101206, China)
Abstract:
In order to explore the population characteristics of plasma prolactin(PRL)concentration in dairy cows and its variation under different environmental conditions and physiological stages, estimate the genetic parameters, and estimate the association between polymorphism of gene PRL, PRLR, TRH and plasma PRL concentration, a total of 1 169 records of plasma PRL concentration from 472 lactating Holstein cows were tested in a large-scale dairy farm in Beijing in 2014 and 2017. Firstly, the effects of sampling season, parity, milking status and lactation stage on plasma PRL concentration were analyzed by employing a fixed model. Then, the genetic parameters of plasma PRL concentration were estimated using a repeatability animal model. In addition, polymorphic loci of gene PRL, PRLR and TRH were screened in Holstein cattle; and the SNP- and haplotype-based association analyses were performed with plasma PRL concentration. The results showed that: 1)The average plasma PRL concentration of Holstein cattle was 224. 29 mIU/L. Parity, sampling season and lactation stage had significant or highly significant impacts on plasma PRL concentration. Plasma PRL concentration gradually increased with the increase of parity and lactation stage, and plasma PRL concentration in spring and summer were extremely significantly higher than that in autumn and winter(P<0. 01); 2)The heritability of plasma PRL concentration was 0. 02, indicating a lowly inheritable nature; 3)Association analyses showed that no SNP within gene PRL and PRLR had significant effect on plasma PRL concentration; rs137596325, rs109144892, rs108991567, rs109468305 and rs109330999 within gene TRH had significant or highly significant effects on plasma PRL concentration. Based on linkage disequilibrium analysis of SNPs in three genes, haplotype block in PRLR gene tended to associate with plasma PRL concentration(P<0. 1), and two haplotype blocks in TRH gene were significantly associated with plasma PRL concentration. In conclusion, this study obtained the population characteristics, genetic parameters and associated genetic markers for plasma PRL concentration in Holstein cows, which was beneficial for further understanding the genetic mechanism of plasma PRL concentration.
Key words:  dairy cattle  plasma prolactin  genetic analysis  PRL  PRLR  TRH