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| 基于ovine SNP 50 K芯片对策勒黑羊繁殖和抗逆性状的遗传性分析 |
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郑浪漫1,2,张璐璐1,2,王若桐1,2,周稳1,2,韩志鹏1,2,刘春洁1,2,王彩军1,2,张成龙1,2,宫昌海3*,刘书东1,2*
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| (1.塔里木大学 动物科学与技术学院, 新疆维吾尔自治区 阿拉尔 843300;2.新疆生产建设兵团塔里木畜牧科技重点实验室, 新疆维吾尔自治区 阿拉尔 843300;3.新疆巴州畜牧工作站, 新疆维吾尔自治区 巴州 841205) |
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| 摘要: |
| 为筛选出策勒黑羊种质资源繁殖和抗逆性状分子标记和相关基因，本研究以100只无亲缘关系的策勒黑羊为研究对象，基于Illumina ovine SNP 50 K bead chip进行基因分型；用Plink1.90对基因组数据进行质量控制；对有效SNPs计算哈代-温伯格值，选择从小到大排列后前1%的分子标记，参考绵羊基因组(Oar_v4.0)进行注释，对注释到的基因进行GO和KEGG分析。结果表明:1)共获得480个SNPs，417个候选基因，其中，繁殖性状基因13个、抗逆性状相关基因30个；2)繁殖性状相关的基因有SOX9、GTF2A1、GNAQ等，抗逆性相关的基因有TMEM154、ZNF70、CREB3L2等。综上，本研究解析了策勒黑羊繁殖和抗逆性状基因的遗传规律，特别是对抗逆性相关候选基因研究，发现策勒黑羊群体携带抗肺炎相关的分子标记，为策勒黑羊保种和改良提供了分子水平的参考。 |
| 关键词: 策勒黑羊 基因组芯片 哈代-温伯格 优异基因 |
| DOI：10.11841/j.issn.1007-4333.2023.01.17 |
| 投稿时间：2022-05-13 |
| 基金项目:国家自然科学基金项目(32060743)；兵团财政科技计划资助(2022CB001-09)；自治区农区高效肉羊品种选育推广技术体系(xjnqry-g-2006)；塔里木大学自治区研究生科研创新项目(TDGRI202133)；大学生创新创业项目(2022018) |
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| Analysis on genetic algorithms of breeding and stress resistance of Qira black sheep based on ovine SNP 50 K bead chip |
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ZHENG Langman1,2,ZHANG Lulu1,2,WANG Ruotong1,2,ZHOU Wen1,2,HAN Zhipeng1,2,LIU Chunjie1,2,WANG Caijun1,2,ZHANG Chenglong1,2,GONG Changhai3*,LIU Shudong1,2*
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| (1.College of Animal Science and Technology, Tarim University, Xinjiang Uygur Autonomous Region, Alar 843300, China;2.Tarim Key Laboratory of Animal Science and Technology, Xinjiang Production and Construction Corps, Xinjiang Uygur Autonomous Region, Alar 843300, China;3.Animal Husbandry Workstation of Xinjiang Bazhou, Xinjiang Uygur Autonomous Region, Bazhou 841205, China) |
| Abstract: |
| In order to screen the molecular markers and related genes of breeding and stress resistance of Qira black sheep germplasm resources, in this study, 100 unrelated Qira black sheep were selected as the research objects. Genotyping was performed based on Illumina ovine SNP 50 K bead chip. Quality control of genomic data was performed with plink1. 90. Hardy-Weinberg values were calculated for the effective SNPs, and the first 1% molecular markers were selected from small to large. The annotated genes were then annotated with reference to the sheep genome(Oar_v4. 0)and these genes were analyzed with GO and KEGG. The results showed that: 1)A total of 480 SNPs and 417 candidate genes were obtained, including 13 reproductive trait genes and 30 stress resistance related genes; 2)The genes related to reproductive traits include SOX9, GTF2A1 and GNAQ etc. and the genes related to stress resistance include TMEM154, ZNF70 and CREB3L2 etc. To sum up, this study analyzed the genetic law of the breeding and stress resistance of Qira black sheep, especially the the candidate genes related to stress resistance. It was found that the Qira black sheep population carried molecular markers related to pneumonia resistance, which provided molecular level reference for the breeding and improvement of Qira black sheep. |
| Key words: Qira black sheep genome chip Hardy-Weinberg excellent genes |
