| 摘要: |
| 为筛选植物工厂环境下白菜型油菜育苗适用铁源并探究铁素浓度对油菜幼苗生长的影响，在铁素浓度2.8 mg/L 下比较了EDDHA-Fe与EDTA-Fe这2种螯合铁的育苗效果；在优选铁源的基础上，设置3个铁素水平(0、2.8和5.6 mg/L)进行油菜育苗，重点调查油菜幼苗根系形态指标、根系活力、茎叶的形态指标以及活性铁含量等。结果表明:1)相同铁素浓度下，利用铁源EDTA-Fe培育的油菜茎叶中活性铁含量较EDDHA-Fe提升了12.6%，具有更高的根系活力；油菜对价格较为低廉的铁源EDTA-Fe的吸收利用效率较高；2)采用优选出的铁源EDTA-Fe进行油菜育苗，相较于2.8 mg/L铁素，铁素浓度为5.6 mg/L时培育的油菜幼苗根系表面积、根系分枝数和根系总长度均得到显著提高，幼苗叶面积提高29.1%，干物质积累量提高18.4%。油菜幼苗在无铁处理组中不能正常生长，侧根发育受阻，未发现二级侧根的形成。综上可知，油菜育苗工厂中铁肥选用EDTA-Fe效益高于EDDHA-Fe，在本试验所设置的环境温湿度及光照条件下，提高铁素浓度至5.6 mg/L更有利于油菜的生长。 |
| 关键词: 白菜型油菜 铁源 活性铁 根系形态 |
| DOI：10.11841/j.issn.1007-4333.2023.01.09 |
| 投稿时间：2022-05-31 |
| 基金项目:国家重点研发计划战略性科技创新合作专项(2020YFE0203600) |
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| Effects of different iron sources and iron concentrations on seedling raising of Brassica campestris |
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LIU Xin,LI Yangmei,JIA Menghan,TONG Yuxin
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| (Institude of Environment and Sustainable Development in Agriculture/Key Laboratory for Energy Saving andWaste Disposal of Protect Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China) |
| Abstract: |
| In order to find out the suitable iron source for Brassica campestris seedling cultivation in plant factory environment and explore the effects of different iron concentrations on the growth of B. campestris seedlings, the seedling raising effects of EDDHA-Fe and EDTA-Fe were firstly compared with the iron concentration of 2. 8 mg/L. After determining the optimum iron source, three iron concentration levels of 0, 2. 8 and 5. 6 mg/L were set up for B. campestris seedling raising. The morphological indexes of root system and shoot, root activity and active iron content of B. campestris seedlings were investigated. The results showed that: 1)Under the same iron concentration, the active iron content in B. campestris shoot cultivated with EDTA-Fe was 12. 6% higher than that with EDDHA-Fe, and the root activity of B. campestris seedlings was also higher with EDTA-Fe; In addition, the Fe absorption and utilization efficiency of B. campestris was high when using EDTA-Fe, which is relatively cheap; 2)The EDTA-Fe was selected as the optimum iron source for the following experiment. Compared to the iron concentration of 2. 8 mg/L, the root surface area, root branch number and total root length of B. campestris seedlings were significantly enhanced under the iron concentration of 5. 6 mg/L. And the leaf area and dry weight of seedling increased by 29. 1% and 18. 4%, respectively. B. campestris seedlings could not grow normally without iron. The development of lateral roots was blocked, and the formation of secondary lateral roots was not found without iron. In conclusion, using EDTA-Fe as iron resource for B. campestris seedling cultivation is better than EDDHA-Fe. Increasing the iron concentration to 5. 6 mg/L is more conducive to the growth of B. campestris in this experiment. |
| Key words: Brassica campestris iron sources activity iron root morphology |
